MCF2/Dbl Antibody

• 产品编号：CST-2089S      品牌：CST       原厂货号：2089S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

MCF2/Dbl Antibody检测内源性MCF2/Dbl总蛋白。基于氨基酸序列的同源，该抗体被期望识别人源MCF2/Dbl蛋白剪切体1-4。该抗体被期望不去识别通过氨基末端的截短的onco-Dbl蛋白形式。通过人工合成人源MCF2/Dbl蛋白氨基末端周围相应多肽片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。

1. Zheng, Y. (2001) Trends Biochem Sci 26, 724-32.
2. Ron, D. et al. (1988) EMBO J 7, 2465-73.
3. Hart, M.J. et al. (1991) Nature 354, 311-4.
4. Kamynina, E. et al. (2007) Mol Cell Biol 27, 1809-22.
5. Batchelor, C.L. et al. (2007) Cell Cycle 6, 353-63.
6. Prag, S. et al. (2007) Mol Biol Cell 18, 2935-48.
7. Vanni, C. et al. (2006) Cell Cycle 5, 2657-65.
8. Morley, S. et al. (2007) Cell Signal 19, 211-8.

MCF2/Dbl原癌基因编码的蛋白是Rho鸟嘌呤核苷交换因子（guanine nucleotide exchange factors,GEFs)的Dbl家族发现的成员，该蛋白是以它们的Dbl homology (DH)结构域为特征(1)。GEFs刺激小GTPases的活性GTP结合形式的形成例如Rho、Rac和Cdc42，这很发信号给不同的下游分子并且调节不同的细胞功能。当Dbl蛋白过表达、全长的Dbl基因有转化活性(2)，突变导致的截短Dbl可引起该蛋白质去变成高度致癌作用。这个Dbl蛋白的截短形式缺少氨基末端497个氨基酸，它是组成GEF活性的要素 (3)，并且比全长变体更加稳定（4）、这允许增加信号到下游效应分子。Dbl蛋白与ezrin蛋白相互作用，而ezrin蛋白是ezrin/radixin/moesin (ERM)家族的成员，它联系细胞质膜到肌动蛋白细胞骨架结构。Dbl蛋白在脂质微区里与ezrin蛋白相互作用，这导致Cdc42 激活和调节相关进程例如丝状伪足形成和细胞极化(5,6)。Dbl蛋白的定位和生物活性在某种程度上通过phosphatidylinositol 3-kinase (PI3K)激酶调节(7)。Dbl蛋白也涉及细胞存活和通过Akt在Thr308位点磷酸化的诱导作用抑制凋亡(8)。

原厂资料：

Specificity / Sensitivity

MCF2/Dbl Antibody recognizes endogenous levels of total MCF2/Dbl protein. Based on amino acid sequence homology, the antibody is expected to recognize splice variants 1-4 of human MCF2/Dbl. The antibody is not expected to recognize the onco-Dbl protein formed through amino-terminal truncation.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human MCF2/Dbl. Antibodies are purified using protein A and peptide affinity chromatography.

Background

The MCF2/Dbl proto-oncogene product is the founding member of the Dbl family of Rho guanine nucleotide exchange factors (GEFs) that are characterized by their Dbl homology (DH) domain (1). GEFs stimulate the formation of the active, GTP-bound form of small GTPases such as Rho, Rac and Cdc42, signaling to various downstream molecules and regulating diverse cell functions. While the overexpressed, full-length Dbl gene has transforming activity (2), mutations resulting in truncated Dbl cause the protein to become highly oncogenic. This truncated form of Dbl, which lacks the amino-terminal 497 amino acids, has constitutive GEF activity (3) and is more stable than the full-length variant (4), allowing for increased signaling to downstream effector molecules. 
 Dbl interacts with ezrin, a member of the ezrin/radixin/moesin (ERM) family of proteins that links the plasma membrane to the actin cytoskeleton. Dbl interacts with ezrin in lipid microdomains, which leads to Cdc42 activation and the regulation of processes such as filopodia formation and cell polarity (5,6). Dbl localization and biological activities are regulated in part by phosphatidylinositol 3-kinase (PI3K) (7). Dbl is also involved in cell survival and inhibits apoptosis through induction of Akt phosphorylation at Thr308 (8).

1. Zheng, Y. (2001) Trends Biochem Sci 26, 724-32.
2. Ron, D. et al. (1988) EMBO J 7, 2465-73.
3. Hart, M.J. et al. (1991) Nature 354, 311-4.
4. Kamynina, E. et al. (2007) Mol Cell Biol 27, 1809-22.
5. Batchelor, C.L. et al. (2007) Cell Cycle 6, 353-63.
6. Prag, S. et al. (2007) Mol Biol Cell 18, 2935-48.
7. Vanni, C. et al. (2006) Cell Cycle 5, 2657-65.
8. Morley, S. et al. (2007) Cell Signal 19, 211-8.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not
aliquot the antibody.