DLK1 Antibody

• 产品编号：CST-2069S      品牌：CST       原厂货号：2069S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

DLK1抗体设别转染水平的DLK1总蛋白.多克隆抗体由合成肽段免疫动物产生，该合成肽段与人DLK1蛋白邻近Met371 氨基酸残基序列一致。抗体由蛋白A和肽段亲和层析技术纯化.

DLK1(delta-like-1),也被认为是胎儿抗原1（FA1）和前细胞因子（pref-1），是表皮生长因子（EGF）样家族蛋白成员之一，含六个串联EGF样重复序列（1,2）。DLK1是父系表达的印记基因，在正常发育和脂肪组织成块的稳态保持在起重要作用（3）。DLK1缺失的小鼠显示生长迟缓，肥胖，骨骼畸形和血清脂代谢增加（4）。已报道DLK1胞外结构域从细胞表面脱落并抑制脂肪分化（5-7）.

1. Laborda, J. et al. (1993) J Biol Chem 268, 3817-20.
2. Smas, C.M. and Sul, H.S. (1993) Cell 73, 725-34.
3. Kobayashi, S. et al. (2000) Genes Cells 5, 1029-37.
4. Moon, Y.S. et al. (2002) Mol Cell Biol 22, 5585-92.
5. Smas, C.M. et al. (1997) Mol Cell Biol 17, 977-88.
6. Mei, B. et al. (2002) Biochem J 364, 137-44.
7. Wang, Y. et al. (2006) J Nutr 136, 2953-6.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Rat

Specificity / Sensitivity

DLK1 Antibody detects transfected levels of total DLK1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a residues surrounding Met371 of human DLK1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

DLK1 (delta-like-1), also known as fetal antigen 1 (FA1) and preadipocyte factor 1 (pref-1), is a member of the epidermal growth factor (EGF)-like family of proteins, containing six tandem EGF-like repeats (1,2). DLK1 is a paternally expressed, imprinted gene that plays an important role in normal development and in the maintenence of homeostasis of adipose tissue mass (3). DLK1 deficient mice display growth retardation, obesity, skeletal malformation, and increased serum lipid metabolites (4). It has been reported that the ectodomain of DLK1 is shredded from the cell surface and inhibits adipocyte differentiation ( 5-7).

1. Laborda, J. et al. (1993) J Biol Chem 268, 3817-20.
2. Smas, C.M. and Sul, H.S. (1993) Cell 73, 725-34.
3. Kobayashi, S. et al. (2000) Genes Cells 5, 1029-37.
4. Moon, Y.S. et al. (2002) Mol Cell Biol 22, 5585-92.
5. Smas, C.M. et al. (1997) Mol Cell Biol 17, 977-88.
6. Mei, B. et al. (2002) Biochem J 364, 137-44.
7. Wang, Y. et al. (2006) J Nutr 136, 2953-6.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody