ENPP1 (L520) Antibody

• 产品编号：CST-2061S      品牌：CST       原厂货号：2061S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

ENPP1 (L520)抗体识别内源性的ENPP1总蛋白水平。该多克隆抗体用与人类ENPP1蛋白中Leu520位点附近的氨基酸序列对应的人工合成肽段免疫动物而制成。该抗体使用蛋白A和蛋白亲和层析纯化而得。

核苷酸内焦磷酸酶-磷酸二酯酶1（ENPP1）是一个单次、II型跨膜蛋白，主要在细胞膜上参与ATP的水解。基底细胞表面ENPP1的瞄定依赖羧基端基于二亮氨酸的信号（1）。ENPP1在骨骼矿化和软组织钙化中扮演重要角色（2-5）。ENPP1基因相应的突变会导致婴儿全身动脉钙化（GACI）和原发性婴儿动脉钙化（IIAC）（6,7）。ENPP1抑制胰岛素受体的功能，过表达时小鼠会发生胰岛素抵抗和葡萄糖不耐受（8,9）。ENPP1基因变异与肥胖和2型糖尿病相关（10-12）。

1. Bello, V. et al. (2001) Mol Biol Cell 12, 3004-15.
2. Okawa, A. et al. (1998) Nat Genet 19, 271-3.
3. Nakamura, I. et al. (1999) Hum Genet 104, 492-7.
4. Harmey, D. et al. (2004) Am J Pathol 164, 1199-209.
5. Hessle, L. et al. (2002) Proc Natl Acad Sci USA 99, 9445-9.
6. Rutsch, F. et al. (2003) Nat Genet 34, 379-81.
7. Ruf, N. et al. (2005) Hum Mutat 25, 98.
8. Dong, H. et al. (2005) Diabetes 54, 367-72.
9. Maddux, B.A. et al. (2006) Am J Physiol Endocrinol Metab 290, E746-9.
10. Meyre, D. et al. (2005) Nat Genet 37, 863-7.
11. Keene, K.L. et al. (2008) Diabetes 57, 1057-62.
12. Bacci, S. et al. (2007) Curr Opin Clin Nutr Metab Care 10, 403-9.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse

Specificity / Sensitivity

ENPP1 (L520) Antibody detects endogenous levels of total ENPP1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu520 of human ENPP1. Antibodies were purified by protein A and peptide affinity chromatography.

Background

Ectonucleotide pyrophosphatase-phosphodiesterase 1 (ENPP1) is a single-pass, type II transmembrane protein primarily involved in ATP hydrolysis at the plasma membrane. Targeting of ENPP1 to the basolateral cell surface relies on the presence of a carboxy-terminal di-leucine-based signal (1). ENPP1 plays important roles in bone mineralization and soft tissue calcification (2-5). Mutations in the corresponding ENPP1 gene cause generalized arterial calcification in infancy (GACI) and idiopathic infantile arterial calcification (IIAC) (6,7). ENPP1 inhibits insulin receptor function and overexpression of this enzyme causes insulin resistance and glucose intolerance in mice (8,9). Genetic variants of ENPP1 have been associated with obesity and type 2 diabetes (10-12).

1. Bello, V. et al. (2001) Mol Biol Cell 12, 3004-15.
2. Okawa, A. et al. (1998) Nat Genet 19, 271-3.
3. Nakamura, I. et al. (1999) Hum Genet 104, 492-7.
4. Harmey, D. et al. (2004) Am J Pathol 164, 1199-209.
5. Hessle, L. et al. (2002) Proc Natl Acad Sci USA 99, 9445-9.
6. Rutsch, F. et al. (2003) Nat Genet 34, 379-81.
7. Ruf, N. et al. (2005) Hum Mutat 25, 98.
8. Dong, H. et al. (2005) Diabetes 54, 367-72.
9. Maddux, B.A. et al. (2006) Am J Physiol Endocrinol Metab 290, E746-9.
10. Meyre, D. et al. (2005) Nat Genet 37, 863-7.
11. Keene, K.L. et al. (2008) Diabetes 57, 1057-62.
12. Bacci, S. et al. (2007) Curr Opin Clin Nutr Metab Care 10, 403-9.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.