PKD/PKCμ Antibody

• 产品编号：CST-2052P      品牌：Cell Signaling Technology       原厂货号：2052P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PKD/PKCmu抗体识别内源性的全部PKD1/PKCmu蛋白。此抗体在某些物种中可能与PKD2亚型有交叉反应。该多克隆抗体用与小鼠PKD中Ser916位点附近的氨基酸序列对应的人工合成磷酸化的肽段免疫动物而制成。该抗体使用蛋白A和蛋白亲和层析纯化而得。

PKC的激活是导致多种细胞反应，如分泌、基因表达、细胞增殖和肌肉收缩的级联反应中的最早期事件之一（1,2）。蛋白激酶D（PKD），也被称为PKCμ，是一种丝氨酸/苏氨酸激酶，其激活依赖于两个活性环位点Ser744和Ser748通过PKC依赖的信号通路的磷酸化（3-5）。除了两个活性环位点，羧基端Ser916位点被确定为PKD/PKCμ自身磷酸化的位点。磷酸化Ser916与PKD/PKCμ的催化活性相关（6）。

1. Nishizuka, Y. (1984) Nature 308, 693-698.
2. Keranen, L.M. (1995) Curr. Biol. 5, 1394-1403.
3. Valverde, A.M. et al. (1994) Proc. Natl. Acad. Sci. 91, 8572-8576.
4. Johannes, F.J. et al. (1994) J. Biol. Chem. 269, 6140-6148.
5. Iglesias, T. et al. (1998) J. Biol. Chem. 273, 27662-27667.
6. Matthews, S.A. et al. (1999) J. Biol. Chem. 274, 26543-26549.
7. Wood, C.D. et al. (2005) J Biol Chem 280, 6245-51.

原厂资料：

Specificity / Sensitivity

PKD/PKCmu Antibody detects endogenous levels of total PKD1/PKCmu protein. This antibody may also react with isoform PKD2, in some species.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser916 of mouse PKD. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Activation of PKC is one of the earliest events in a cascade leading to a variety of cellular responses such as secretion, gene expression, proliferation and muscle contraction (1,2). Protein kinase D (PKD), also called PKCμ, is a serine/threonine kinase whose activation is dependent on the phosphorylation of two activation loop sites, Ser744 and Ser748, via a PKC-dependent signaling pathway (3-5). In addition to the two activation loop sites, the carboxy-terminal Ser916 has been identified as an autophosphorylation site for PKD/PKCμ. Phosphorylation at Ser916 correlates with PKD/PKCμ catalytic activity (6).

1. Nishizuka, Y. (1984) Nature 308, 693-698.
2. Keranen, L.M. (1995) Curr. Biol. 5, 1394-1403.
3. Valverde, A.M. et al. (1994) Proc. Natl. Acad. Sci. 91, 8572-8576.
4. Johannes, F.J. et al. (1994) J. Biol. Chem. 269, 6140-6148.
5. Iglesias, T. et al. (1998) J. Biol. Chem. 273, 27662-27667.
6. Matthews, S.A. et al. (1999) J. Biol. Chem. 274, 26543-26549.
7. Wood, C.D. et al. (2005) J Biol Chem 280, 6245-51.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.