Tug Antibody

• 产品编号：CST-2049S      品牌：CST       原厂货号：2049S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Tug抗体识别内源性的Tug总蛋白水平。该多克隆抗体用与人类Tug蛋白Val542附近的氨基酸链对应的人工合成肽段免疫动物而制成。该抗体使用蛋白A和蛋白亲和层析纯化而得。

Tug（含UBX结构域的Glut4栓系蛋白），又称ASPL，ASPSCR1，RCC17，UBXD9，UBXN9，首次发现于腺泡状软组织肉瘤患者，是一个TFE3染色体易位伴侣，在其C-端区域包含一个UBX类似域。Tug在细胞内囊泡中与Glut4栓系，在胰岛素刺激下转位释放Glut4到细胞表面。稳定的Tug缺陷或显性失活将刺激Glut4重新分布（3）。

1. Ladanyi, M. et al. (2001) Oncogene 20, 48-57.
2. Bogan, J.S. et al. (2003) Nature 425, 727-33.
3. Yu, C. et al. (2007) J Biol Chem 282, 7710-22.

原厂资料：

Specificity / Sensitivity

Tug Antibody detects endogenous levels of total Tug protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids around residue Val542 of human Tug. Antibodies are purified by Protein A and peptide affinity chromatography.

Background

Tug (Tether containing UBX domain for GLUT4), also known as ASPL, ASPSCR1, RCC17, UBXD9, UBXN9, was first identified as a chromosomal translocation partner for TFE3 in patients with Alveolar soft part sarcoma (1) and contains an UBX-like domain in its C-terminal region. Tug is found to tether GLUT4 in intracellular vesicles and to release GLUT4 for cell surface translocation upon insulin stimulation (2). Stable Tug depletion or expression of a dominant negative form stimulates GLUT4 redistribution (3).

1. Ladanyi, M. et al. (2001) Oncogene 20, 48-57.
2. Bogan, J.S. et al. (2003) Nature 425, 727-33.
3. Yu, C. et al. (2007) J Biol Chem 282, 7710-22.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.