PP2A A Subunit (81G5) Rabbit mAb

• 产品编号：CST-2041S      品牌：CST       原厂货号：2041S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PP2A A Subunit (81G5) Rabbit mAb能够检测内源性PP2A A亚基，包括α和β单体。该抗体不与其他PP2A亚基发生交叉反应。该单克隆抗体是由合成的人源的针对PP2A A亚基蛋白免疫动物生产的。

蛋白磷酸酶2A (PP2A)是一种重要的蛋白丝氨酸/苏氨酸磷酸酶，在所有的真核细胞中结构保守。PP2A在各种细胞信号转导通路中都是关键的酶，它调节基本的细胞活动如DNA复制、转录、翻译、代谢、细胞周期进程、细胞分化、细胞凋亡和发育(1-3)。核心的酶由催化性的C亚基和调节性的A亚基(PR65)组成，每个亚基都包括α 和β异构体(1)。另外的亚基属于四个不相关蛋白的不同家族。B (PR55)和B'调节蛋白家族都包含α，β，γ 和δ异构体，而B'还含有ε蛋白。B''家族包括PR72，PR130，PR59 和PR48异构体，而纹蛋白(PR110)和SG2NA (PR93)都属于B'''调节蛋白家族。这些B亚基竞争性的结合核心亚基A上面的一个共同结合位点(1)。该全酶的可变阵列，尤其是调节性B亚基，允许PP2A具有多种功能。PP2A的功能受其表达、分布、全酶构成和转录后修饰的调控。Src介导的PP2A第307位酪氨酸磷酸化响应EGF或者胰岛素，最终导致PP2A的活性显著下降(4)。已观察到PP2A的第309位亮氨酸的羧基官能团可发生可逆的甲基化修饰(5,6)。甲基化改变了PP2A的构象，也改变了它的定位和B调节亚基的相互作用(6-8)。

1. Janssens, V. and Goris, J. (2001) Biochem. J. 353, 417-439.
2. Zolnierowicz, S. (2000) Biochem. Pharmacol. 60, 1225-1235.
3. Milward, T.A. et al. (1999) Trends Biochem. Sci. 24, 186-191.
4. Chen, J. et al. (1992) Science 257, 1261-1264.
5. Turowski, P. et al. (1995) J. Cell. Biol. 129, 397-410.
6. Lee, J. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 6043-6047.
7. Tolstykh, T. et al. (2000) EMBO J. 19, 5682-5691.
8. Yu, X.X. et al. (2001) Mol. Biol. Cell 12, 185-199.

原厂资料：

Specificity / Sensitivity

PP2A A Subunit (81G5) Rabbit mAb detects endogenous levels of PP2A A subunit, both α and β isoforms. The antibody does not cross-react with other PP2A subunits.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PP2A A Subunit protein.

Background

Protein phosphatase type 2A (PP2A) is an essential protein serine/threonine phosphatase that is conserved in all eukaryotes. PP2A is a key enzyme within various signal transduction pathways as it regulates fundamental cellular activities such as DNA replication, transcription, translation, metabolism, cell cycle progression, cell division, apoptosis and development (1-3). The core enzyme consists of catalytic C and regulatory A (or PR65) subunits, with each subunit represented by α and β isoforms (1). Additional regulatory subunits belong to four different families of unrelated proteins. Both the B (or PR55) and B' regulatory protein families contain α, β, γ and δ isoforms, with the B' family also including an ε protein. B'' family proteins include PR72, PR130, PR59 and PR48 isoforms, while striatin (PR110) and SG2NA (PR93) are both members of the B''' regulatory protein family. These B subunits competitively bind to a shared binding site on the core A subunit (1). This variable array of holoenzyme components, particularly regulatory B subunits, allows PP2A to act in a diverse set of functions. PP2A function is regulated by expression, localization, holoenzyme composition and post-translational modification. Phosphorylation of PP2A at Tyr307 by Src occurs in response to EGF or insulin and results in a substantial reduction of PP2A activity (4). Reversible methylation on the carboxyl group of Leu309 of PP2A has been observed (5,6). Methylation alters the conformation of PP2A, as well as its localization and association with B regulatory subunits (6-8). 

1. Janssens, V. and Goris, J. (2001) Biochem. J. 353, 417-439.
2. Zolnierowicz, S. (2000) Biochem. Pharmacol. 60, 1225-1235.
3. Milward, T.A. et al. (1999) Trends Biochem. Sci. 24, 186-191.
4. Chen, J. et al. (1992) Science 257, 1261-1264.
5. Turowski, P. et al. (1995) J. Cell. Biol. 129, 397-410.
6. Lee, J. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 6043-6047.
7. Tolstykh, T. et al. (2000) EMBO J. 19, 5682-5691.
8. Yu, X.X. et al. (2001) Mol. Biol. Cell 12, 185-199.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%so-
dium azide. Store at –20°C. Do not aliquot the antibody