Lamin A/C Antibody

• 产品编号：CST-2032S      品牌：CST       原厂货号：2032S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Lamin A/C Antibody 能够检测内源的全长lamin A（和lamin C）蛋白(70 kDa)。它也能够检测在Asp230位点酶切的lamin A（和C 蛋白）的小片段。该多克隆抗体是采用合成的人源lamin A/C蛋白sp230周围残基相对应的肽段免疫动物而制备的。体由protein A和肽亲和层析技术纯化。Lamin是核膜的结构组分，对维持正常的细胞功能非常重要，比如细胞周期调控、DNA修复和染色质组装（1-3）。Lamin A/C能够被caspase-6切割，可以作为caspase-6激活的标志。在凋亡过程中，Lamin A/C能够被特异地剪切成大片段(41-50 kDa)和小片段 (28 kDa)（3,4）。Lamin的剪切会引起细胞核的失调和细胞死亡（5，6）。

1. Gruenbaum, Y. et al. (2000) J Struct Biol 129, 313-23.
2. Yabuki, M. et al. (1999) Physiol Chem Phys Med NMR 31, 77-84.
3. Goldberg, M. et al. (1999) Crit Rev Eukaryot Gene Expr 9, 285-93.
4. Orth, K. et al. (1996) J Biol Chem 271, 16443-6.
5. Oberhammer, F.A. et al. (1994) J Cell Biol 126, 827-37.
6. Rao, L. et al. (1996) J Cell Biol 135, 1441-55.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Bovine

Specificity / Sensitivity

Lamin A/C Antibody detects endogenous levels of total full length lamin A (and lamin C) (70 kDa), as well as the small fragment of lamin A (and lamin C) resulting from cleavage at aspartic acid 230 (28 kDa).

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp230 of human lamin A. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication and chromatin organization (1-3). Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into a large (41-50 kDa) and a small (28 kDa) fragment (3,4). The cleavage of lamins results in nuclear disregulation and cell death (5,6).

1. Gruenbaum, Y. et al. (2000) J Struct Biol 129, 313-23.
2. Yabuki, M. et al. (1999) Physiol Chem Phys Med NMR 31, 77-84.
3. Goldberg, M. et al. (1999) Crit Rev Eukaryot Gene Expr 9, 285-93.
4. Orth, K. et al. (1996) J Biol Chem 271, 16443-6.
5. Oberhammer, F.A. et al. (1994) J Cell Biol 126, 827-37.
6. Rao, L. et al. (1996) J Cell Biol 135, 1441-55.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.