MCP-1 Antibody (Mouse Specific)

• 产品编号：CST-2029S      品牌：CST       原厂货号：2029S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

MCP-1 Antibody(Mouse Specific)能够检测内源水平鼠MCP-1蛋白总体水平。此多克隆抗体是通过合成鼠源对应的MCP-1 Cys75位点周围的肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。

单核细胞趋化蛋白-1 (MCP-1), 也叫CCL2, 单核细胞趋化激活因子(MCAF)或者神经胶质瘤源性趋化因子-2 (GDCF-2)，是人JE基因的产物，并且是C-C(或β)趋化因子家族的成员之一(1-4)。MCP-1蛋白的预测分子量是11-13 kDa, 但是由于糖基化作为20-30 kDa蛋白而迁移。在对促炎症因子刺激的应答中，很多细胞类型都能分泌MCP-1蛋白，它最初是对单核细胞的趋化性中描述。此活性促进了对它的研究，并阐明其在单核细胞浸润疾病如牛皮癣(5)、类风湿性关节炎 (6)和动脉粥样硬化(7)分类中的作用。MCP-1也对肿瘤进展和血管生成有作用(8)。MCP-1的信号是通过G蛋白偶联受体CCR2介导(9)。

1. Matsushima, K. et al. (1989) J Exp Med 169, 1485-90.
2. Furutani, Y. et al. (1989) Biochem Biophys Res Commun 159, 249-55.
3. Robinson, E.A. et al. (1989) Proc Natl Acad Sci USA 86, 1850-4.
4. Rollins, B.J. et al. (1988) Proc Natl Acad Sci USA 85, 3738-42.
5. Gillitzer, R. et al. (1993) J Invest Dermatol 101, 127-31.
6. Koch, A.E. et al. (1992) J Clin Invest 90, 772-9.
7. Ylä-Herttuala, S. et al. (1991) Proc Natl Acad Sci USA 88, 5252-6.
8. Salcedo, R. et al. (2000) Blood 96, 34-40.
9. Charo, I.F. et al. (1994) Proc Natl Acad Sci USA 91, 2752-6.

原厂资料：

Specificity / Sensitivity

MCP-1 Antibody (Mouse Specific) detects endogenous levels of total mouse MCP-1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Cys75 of mouse MCP-1. Antibodies were purified by protein A and peptide affinity chromatography.

Background

Monocyte chemotactic protein-1 (MCP-1), also known as CCL2, monocyte chemotactic activating factor (MCAF) or glioma-derived chemotactic factor-2 (GDCF-2), is the product of the human JE gene and a member of the family of C-C (or β) chemokines (1-4). The predicted molecular weight of MCP-1 protein is 11-13 kDa, but it may migrate at 20-30 kDa due to glycosylation. MCP-1 is secreted by a variety of cell types in response to pro-inflammatory stimuli and was originally described for its chemotactic activity on monocytes. This activity has led to studies demonstrating its role in diseases characterized by monocyte infiltrates such as psoriasis (5), rheumatoid arthritis (6) and atherosclerosis (7). MCP-1 may also contribute to tumor progression and angiogenesis (8). Signaling by MCP-1 is mediated by the G-protein coupled receptor CCR2 (9).

1. Matsushima, K. et al. (1989) J Exp Med 169, 1485-90.
2. Furutani, Y. et al. (1989) Biochem Biophys Res Commun 159, 249-55.
3. Robinson, E.A. et al. (1989) Proc Natl Acad Sci USA 86, 1850-4.
4. Rollins, B.J. et al. (1988) Proc Natl Acad Sci USA 85, 3738-42.
5. Gillitzer, R. et al. (1993) J Invest Dermatol 101, 127-31.
6. Koch, A.E. et al. (1992) J Clin Invest 90, 772-9.
7. Ylä-Herttuala, S. et al. (1991) Proc Natl Acad Sci USA 88, 5252-6.
8. Salcedo, R. et al. (2000) Blood 96, 34-40.
9. Charo, I.F. et al. (1994) Proc Natl Acad Sci USA 91, 2752-6.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.