FoxD3 (D20A9) Rabbit mAb

• 产品编号：CST-2019S      品牌：CST       原厂货号：2019S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

FoxD3 (D20A9)兔源单抗识别转染水平的的总FoxD3 蛋白.此单克隆抗体由合成肽段免疫动物产生，该肽段与人FoxD3的羧基末端氨基酸残基序列一致.FoxD3是叉头盒家族成员之一，以翼型螺旋DNA结合结构和胚胎发育中的重要作用为特征（1）。该转录调节因子，对于小鼠胚胎发育的植入前和着床期多潜能的维持是必需的（2），它也是滋养层细胞形成所必需的（3）。FoxD3对哺乳动物神经嵴的保持是必需的，FoxD3(-/-)小鼠胚胎神经嵴衍生的结构缺失，不能在植入后存活（4）。FoxD3也通过Oct-4 和NANOG形成调控网络保持ES细胞多潜能性（5,6）.

1. Sutton, J. et al. (1996) J Biol Chem 271, 23126-33.
2. Hanna, L.A. et al. (2002) Genes Dev 16, 2650-61.
3. Tompers, D.M. et al. (2005) Dev Biol 285, 126-37.
4. Teng, L. et al. (2008) Development 135, 1615-24.
5. Pan, G. et al. (2006) FASEB J 20, 1730-2.
6. Liu, Y. and Labosky, P.A. (2008) Stem Cells 26, 2475-84.

原厂资料：

Specificity / Sensitivity

FoxD3 (D20A9) Rabbit mAb detects transfected levels of total FoxD3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues at the C-terminus of human FoxD3.

Background

FoxD3 is a member of the Forkhead Box family and is characterized by a winged-helix DNA-binding structure and the important role it plays in embryonic development (1). This transcriptional regulator is required for the maintenance of pluripotency in the pre-implantation and peri-implantation stages of mouse embryonic development (2) and is also required for trophoblast formation (3). FoxD3 is required for the maintenance of the mammalian neural crest; FoxD3(-/-) mouse embryos fail around the time of implantation with loss of neural crest-derived structures (4). FoxD3 also forms a regulatory network with Oct-4 and NANOG to maintain the pluripotency of ES cells (5,6).

1. Sutton, J. et al. (1996) J Biol Chem 271, 23126-33.
2. Hanna, L.A. et al. (2002) Genes Dev 16, 2650-61.
3. Tompers, D.M. et al. (2005) Dev Biol 285, 126-37.
4. Teng, L. et al. (2008) Development 135, 1615-24.
5. Pan, G. et al. (2006) FASEB J 20, 1730-2.
6. Liu, Y. and Labosky, P.A. (2008) Stem Cells 26, 2475-84.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody