BID Antibody (Human Specific)

• 产品编号：CST-2002S      品牌：CST       原厂货号：2002S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

该抗体能够检测内源的人 BID 全长(22 kDa)蛋白和裂解大片段 (15 kDa)。该多克隆抗体是用针对人源BID裂解位点周围氨基酸序列合成肽免疫动物后制备获得。抗体通过蛋白A和肽段亲和层析而纯化.

含有BH3结构域的BID蛋白，是Bcl-2/Bcl-xL家族的一种死亡激动剂成员(1)，以钝化前体形式分布在细胞质中(2,3)。在Fas信号通路中，其被caspase-8裂解并活化。裂解的BID片段转位至线粒体，并诱导细胞色素C释放和线粒体的损伤(2-5)。因此，BID传递从细胞表面到线粒体的凋亡信号。然而，凋亡过程中裂解的BID片段精确的转位及引发的细包色素c的释放机制尚未阐明。

1. Wang, K. et al. (1996) Genes Dev 10, 2859-69.
2. Luo, X. et al. (1998) Cell 94, 481-90.
3. Li, H. et al. (1998) Cell 94, 491-501.
4. Gross, A. et al. (1999) J Biol Chem 274, 1156-63.
5. Li, P. et al. (1997) Cell 91, 479-89.
6. Zou, H. et al. (1999) J Biol Chem 274, 11549-56.
7. Saleh, A. et al. (1999) J Biol Chem 274, 17941-5.
8. Yin, X.M. et al. (1999) Nature 400, 886-91.
9. Korsmeyer, S.J. et al. (2000) Cell Death Differ 7, 1166-73.

原厂资料：

Specificity / Sensitivity

BID Antibody (Human Specific) detects endogenous levels of both the full length (22 kDa) and cleaved large fragment (15 kDa) of human BID.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the cleavage site of human BID. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The BH3 domain-only protein, BID, a death agonist member of the Bcl-2/Bcl-xL family (1), is localized in the cytosolic fraction of cells as an inactive precursor (2,3). Its active form is generated upon proteolytic cleavage by caspase-8 in the Fas signaling pathway. Cleaved BID translocates to mitochondria and induces cytochrome c release and mitochondrial damage (2-5). Thus, BID relays an apoptotic signal from the cell surface to mitochondria. However, the precise molecular mechanism for the translocation of the cleaved BID, and for the subsequent release of cytochrome c during apoptosis, is still unclear.

1. Wang, K. et al. (1996) Genes Dev 10, 2859-69.
2. Luo, X. et al. (1998) Cell 94, 481-90.
3. Li, H. et al. (1998) Cell 94, 491-501.
4. Gross, A. et al. (1999) J Biol Chem 274, 1156-63.
5. Li, P. et al. (1997) Cell 91, 479-89.
6. Zou, H. et al. (1999) J Biol Chem 274, 11549-56.
7. Saleh, A. et al. (1999) J Biol Chem 274, 17941-5.
8. Yin, X.M. et al. (1999) Nature 400, 886-91.
9. Korsmeyer, S.J. et al. (2000) Cell Death Differ 7, 1166-73.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.