此种生物素化的肽段包含谷氨酸(Glu)和酪氨酸(Tyr),其比例为4:1,此生物素化的肽段在酪氨酸激酶实验中作为底物,也可以在酪氨酸磷酸酶实验中作为阳性对照。此种生物素化的肽段的品质是采用逆向HOLC和质谱分析法评估的。肽段的磷酸化形式可以用Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411检测。附上样本激酶和磷酸酶分析的实验步骤。
原厂资料:
Description
This biotinylated peptide contains glutamic acid (Glu) and tyrosine (Tyr) at a ratio of 4:1 (Glu:Tyr). This biotinylated peptide was generated for use as substrate in a tyrosine kinase assays, and also can serve as a positive control in tyrosine phosphatase assays.
Quality Control
The quality of the biotinylated peptide was evaluated by reverse-phase HPLC and by mass spectrometry.
Directions for Use
The phosphorylated form of the peptide can be detected with the Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411. Sample kinase and phosphatase assay protocols are attached.
注意事项:
Storage: Supplied in 0.0001% DMSO. Store at -20°C.