RPL5 Antibody

• 产品编号：CST-14568S      品牌：CST       原厂货号：14568S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Homology

Species predicted to react based on 100% sequence homology: Bovine, Pig, Horse

Specificity / Sensitivity

RPL5 Antibody recognizes endogenous levels of total RPL5 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln122 of human RPL5 protein. Antibodies are purified by protein A and peptide affinity chromatography.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Bovine, Pig, Horse

Specificity / Sensitivity

RPL5 Antibody recognizes endogenous levels of total RPL5 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln122 of human RPL5 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Ribosomal protein L5 (RPL5) is one of several proteins that comprise the 60S ribosomal subunit. RPL5 binds 5S rRNA and the nucleolar RPL11 protein to form the 5S ribonucleoprotein particle (RNP) that is incorporated into the large 60S ribosomal subunit (1). An RP-MDM2-p53 protein complex that contains ribosomal proteins RPL5, RPL11, and RPL23 acts as a nucleolar stress sensor that binds and inhibits MDM2 ubiquitin ligase activity and enhances p53-mediated transcriptional activity (2,3). RPL5 cooperates with RPL11 to influence ribosome biogenesis through regulating expression of the transcription factor c-Myc, which acts as the master regulator of ribosome biogenesis (4). Mutations in the corresponding RPL5 gene are associated with Diamond-Blackfan anemia, which is a form of red blood cell aplasia, and some cases of pediatric T-cell acute lymphoblastic leukemia (5,6).

1. Zhang, J. et al. (2007) Genes Dev 21, 2580-92.
2. Horn, H.F. and Vousden, K.H. (2008) Oncogene 27, 5774-84.
3. Dai, M.S. and Lu, H. (2004) J Biol Chem 279, 44475-82.
4. Liao, J.M. et al. (2013) Oncogene, [Epub ahead of print].
5. Avondo, F. et al. (2009) BMC Genomics 10, 442.
6. De Keersmaecker, K. et al. (2013) Nat Genet 45, 186-90.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not
aliquot the antibody.