Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb (PE Conjugate)

• 产品编号：CST-14566S      品牌：CST       原厂货号：14566S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Specificity / Sensitivity

Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb (PE Conjugate) detects endogenous levels of cdc2 protein only when phosphorylated at Tyr15. Based on sequence similarity, the antibody may cross-react with CDK2 and CDK3.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr15 of human cdc2.

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb #4539.

原厂资料：

Specificity / Sensitivity

Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb (PE Conjugate) detects endogenous levels of cdc2 protein only when phosphorylated at Tyr15. Based on sequence similarity, the antibody may cross-react with CDK2 and CDK3.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr15 of human cdc2.

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb #4539.

Background

The entry of eukaryotic cells into mitosis is regulated by cdc2 kinase activation, a process controlled at several steps including cyclin binding and phosphorylation of cdc2 at Thr161 (1). However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Thr14 and Tyr15 (2). Phosphorylation at Thr14 and Tyr15, resulting in inhibition of cdc2, can be carried out by Wee1 and Myt1 protein kinases (3,4). The cdc25 phosphatase may be responsible for removal of phosphates at Thr14 and Tyr15 and subsequent activation of cdc2 (1,5).

1. Atherton-Fessler, S. et al. (1994) Mol Biol Cell 5, 989-1001.
2. Norbury, C. et al. (1991) EMBO J 10, 3321-9.
3. McGowan, C.H. and Russell, P. (1993) EMBO J 12, 75-85.
4. Wells, N.J. et al. (1999) J Cell Sci 112 ( Pt 19), 3361-71.
5. Hunter, T. (1995) Cell 80, 225-36.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the
antibodies. Protect from light. Do not freeze.