Phospho-MLKL (Thr357/Ser358) Antibody

• 产品编号：CST-14516S      品牌：Cell Signaling Technology       原厂货号：14516S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Specificity / Sensitivity

Phospho-MLKL (Thr357/Ser358) recognizes endogenous levels of MLKL protein only when phosphorylated at Thr357 and Ser358. This antibody can recognize single phosphorylation sites at Thr357 or Ser358, or when dually phosphorylated at both sites.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr357/Ser358 of human MLKL protein. Antibodies are purified by protein A and peptide affinity chromatography.

原厂资料：

Specificity / Sensitivity

Phospho-MLKL (Thr357/Ser358) recognizes endogenous levels of MLKL protein only when phosphorylated at Thr357 and Ser358. This antibody can recognize single phosphorylation sites at Thr357 or Ser358, or when dually phosphorylated at both sites.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr357/Ser358 of human MLKL protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Necroptosis, a regulated pathway for necrotic cell death, is triggered by a number of inflammatory signals including cytokines in the tumor necrosis factor (TNF) family, pathogen sensors such as toll-like receptors (TLRs), and ischemic injury (1,2). The process is negatively regulated by caspases and is initiated through a complex containing the RIP1 and RIP3 kinases, typically referred to as the necrosome. Mixed lineage kinase domain-like protein (MLKL) is a pseudokinase that was identified as downstream target of RIP3 in the necroptosis pathway (3,4). During necroptosis RIP3 is phosphorylated at Ser227, which recruits MLKL and leads to its phosphorylation at Thr357 and Ser358 (3). Knockdown of MLKL through multiple mechanisms results in inhibition of necroptosis (3-5). While the precise mechanism for MLKL-induced necroptosis is unclear, some studies have shown that necroptosis leads to oligomerization of MLKL and translocation to the plasma membrane, where it effects membrane integrity (6-9).

1. Christofferson, D.E. and Yuan, J. (2010) Curr Opin Cell Biol 22, 263-8.
2. Kaczmarek, A. et al. (2013) Immunity 38, 209-23.
3. Sun, L. et al. (2012) Cell 148, 213-27.
4. Wang, Z. et al. (2012) Cell 148, 228-43.
5. Wu, J. et al. (2013) Cell Res 23, 994-1006.
6. Cai, Z. et al. (2014) Nat Cell Biol 16, 55-65.
7. Chen, X. et al. (2014) Cell Res 24, 105-21.
8. Wang, H. et al. (2014) Mol Cell 54, 133-46.
9. Dondelinger, Y. et al. (2014) Cell Rep 7, 971-81.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.