NRF2 (D1Z9C)?XP??Rabbit mAb?(PE Conjugate)

• 产品编号：CST-14409S      品牌：CST       原厂货号：14409S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Specificity / Sensitivity

NRF2 (D1Z9C) XP^® Rabbit mAb (PE Conjugate) recognizes endogenous levels of total NRF2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala275 of human NRF2 protein.

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated NRF2 (D1Z9C) XP^® Rabbit mAb #12721.

原厂资料：

Specificity / Sensitivity

NRF2 (D1Z9C) XP^® Rabbit mAb (PE Conjugate) recognizes endogenous levels of total NRF2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala275 of human NRF2 protein.

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated NRF2 (D1Z9C) XP^® Rabbit mAb #12721.

Background

The nuclear factor-like 2 (NRF2) transcriptional activator binds antioxidant response elements (ARE) of target gene promoter regions to regulate expression of oxidative stress response genes. Under basal conditions, the NRF2 inhibitor INrf2 (also called KEAP1) binds and retains NRF2 in the cytoplasm where it can be targeted for ubiquitin-mediated degradation (1). Small amounts of constitutive nuclear NRF2 maintain cellular homeostasis through regulation of basal expression of antioxidant response genes. Following oxidative or electrophilic stress, KEAP1 releases NRF2, thereby allowing the activator to translocate to the nucleus and bind to ARE-containing genes (2). The coordinated action of NRF2 and other transcription factors mediates the response to oxidative stress (3). Altered expression of NRF2 is associated with chronic obstructive pulmonary disease (COPD) (4). NRF2 activity in lung cancer cell lines directly correlates with cell proliferation rates, and inhibition of NRF2 expression by siRNA enhances anti-cancer drug-induced apoptosis (5).

1. Cullinan, S.B. et al. (2004) Mol Cell Biol 24, 8477-86.
2. Nguyen, T. et al. (2005) J Biol Chem 280, 32485-92.
3. Jaiswal, A.K. (2004) Free Radic Biol Med 36, 1199-207.
4. Suzuki, M. et al. (2008) Am J Respir Cell Mol Biol 39, 673-82.
5. Homma, S. et al. (2009) Clin Cancer Res 15, 3423-32.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the
antibodies. Protect from light. Do not freeze.