RPL11 Antibody

• 产品编号：CST-14382S      品牌：Cell Signaling Technology       原厂货号：14382S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Homology

Species predicted to react based on 100% sequence homology: Chicken, Bovine, Pig

Specificity / Sensitivity

RPL11 Antibody recognizes endogenous levels of total RPL11 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human RPL11 protein. Antibodies are purified by protein A and peptide affinity chromatography.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Chicken, Bovine, Pig

Specificity / Sensitivity

RPL11 Antibody recognizes endogenous levels of total RPL11 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human RPL11 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Ribosomal protein L11 (RPL11) is a nucleolar protein and component of the 60S ribosomal subunit. Research studies have shown that RPL11 plays a critical role in eukaryotic ribosome biogenesis (1). It has also been suggested that extraribosomal RPL11 functions in a RP-MDM2-p53 network in which RPL11 acts as a sensor of nucleolar stresses that perturb ribosome biogenesis (2). Indeed, RPL11 contributes to enhanced p53 stability and transcriptional activity in response to nucleolar stress and impaired ribosome biogenesis by binding and inhibiting the ubiquitin ligase activity of MDM2 (2-9). In addition to regulating p53 activity, research studies have also shown that RPL11 can inhibit cell cycle progession and ribosome biogenesis in response to nucleolar stress through repression of c-Myc transcriptional activity as well as its mRNA translation (10-12). Mutations in the RPL11 gene have been found in patients with Diamond-Blackfan anemia (13).

1. Zhang, J. et al. (2007) Genes Dev 21, 2580-92.
2. Horn, H.F. and Vousden, K.H. (2008) Oncogene 27, 5774-84.
3. Zhou, X. et al. (2013) Oncogene 32, 388-96.
4. Bursać, S. et al. (2012) Proc Natl Acad Sci U S A 109, 20467-72.
5. Fumagalli, S. et al. (2009) Nat Cell Biol 11, 501-8.
6. Lohrum, M.A. et al. (2003) Cancer Cell 3, 577-87.
7. Zhang, Y. et al. (2003) Mol Cell Biol 23, 8902-12.
8. Sun, X.X. et al. (2010) J Biol Chem 285, 25812-21.
9. Mahata, B. et al. (2012) Oncogene 31, 3060-71.
10. Dai, M.S. et al. (2010) J Biol Chem 285, 12587-94.
11. Dai, M.S. et al. (2007) EMBO J 26, 3332-45.
12. Challagundla, K.B. et al. (2011) Mol Cell Biol 31, 4007-21.
13. Gazda, H.T. et al. (2008) Am J Hum Genet 83, 769-80.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not
aliquot the antibody.