Phospho-GSK-3β (Ser9) (D85E12) XP??Rabbit mAb?(Pacific Blue^? Conjugate)

• 产品编号：CST-14310S      品牌：CST       原厂货号：14310S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Specificity / Sensitivity

Phospho-GSK-3β (Ser9) (D85E12) XP^® Rabbit mAb (Pacific Blue^™ Conjugate) detects endogenous levels of GSK-3β only when phosphorylated at Ser9. This antibody reacts with denatured components of bovine serum, including BSA.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser9 of human GSK-3β.

Description

This Cell Signaling Technology antibody is conjugated to Pacific Blue^™ fluorescent dye and tested in-house for direct flow cytometry in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated antibody Phospho-GSK-3β (Ser9) (D85E12) XP^® Rabbit mAb #5558.

原厂资料：

Specificity / Sensitivity

Phospho-GSK-3β (Ser9) (D85E12) XP^® Rabbit mAb (Pacific Blue^™ Conjugate) detects endogenous levels of GSK-3β only when phosphorylated at Ser9. This antibody reacts with denatured components of bovine serum, including BSA.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser9 of human GSK-3β.

Description

This Cell Signaling Technology antibody is conjugated to Pacific Blue^™ fluorescent dye and tested in-house for direct flow cytometry in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated antibody Phospho-GSK-3β (Ser9) (D85E12) XP^® Rabbit mAb #5558.

Background

Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).

1. Welsh, G.I. et al. (1996) Trends Cell Biol 6, 274-9.
2. Srivastava, A.K. and Pandey, S.K. (1998) Mol Cell Biochem 182, 135-41.
3. Cross, D.A. et al. (1995) Nature 378, 785-9.
4. Nusse, R. (1997) Cell 89, 321-3.
5. Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the
antibody. Protect from light. Do not freeze.