Phospho-CREB (Ser133) (87G3) Rabbit mAb?(PE?Conjugate)

• 产品编号：CST-14228S      品牌：CST       原厂货号：14228S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Homology

Species predicted to react based on 100% sequence homology: Zebrafish

Specificity / Sensitivity

Phospho-CREB (Ser133) (87G3) Rabbit mAb (PE Conjugate) detects endogenous levels of CREB only when phosphorylated at Ser133. The antibody also detects the phosphorylated form of the CREB-related protein, ATF-1.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser133 of human CREB protein.

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-CREB (Ser133) (87G3) Rabbit mAb #9198.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Zebrafish

Specificity / Sensitivity

Phospho-CREB (Ser133) (87G3) Rabbit mAb (PE Conjugate) detects endogenous levels of CREB only when phosphorylated at Ser133. The antibody also detects the phosphorylated form of the CREB-related protein, ATF-1.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser133 of human CREB protein.

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-CREB (Ser133) (87G3) Rabbit mAb #9198.

Background

CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca²⁺, and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2 (7-9).

1. Lonze, B.E. et al. (2002) Neuron 34, 371-85.
2. Lee, M.M. et al. (1999) J Neurosci Res 55, 702-12.
3. Redmond, L. et al. (2002) Neuron 34, 999-1010.
4. Dash, P.K. et al. (1990) Nature 345, 718-21.
5. Yin, J.C. et al. (1994) Cell 79, 49-58.
6. Guzowski, J.F. and McGaugh, J.L. (1997) Proc Natl Acad Sci USA 94, 2693-8.
7. Xing, J. et al. (1998) Mol Cell Biol 18, 1946-55.
8. Ribar, T.J. et al. (2000) J Neurosci 20, RC107.
9. Tan, Y. et al. (1996) EMBO J 15, 4629-42.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the
antibodies. Protect from light. Do not freeze.