CDK2 (78B2) Rabbit mAb (PE Conjugate)

• 产品编号：CST-14174S      品牌：CST       原厂货号：14174S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Specificity / Sensitivity

CDK2 (78B2) Rabbit mAb (PE Conjugate) detects endogenous levels of total CDK2 protein. The unconjugated antibody does not cross-react with other CDK proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues of human CDK2 protein.

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated CDK2 (78B2) Rabbit mAb #2546.

原厂资料：

Specificity / Sensitivity

CDK2 (78B2) Rabbit mAb (PE Conjugate) detects endogenous levels of total CDK2 protein. The unconjugated antibody does not cross-react with other CDK proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues of human CDK2 protein.

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated CDK2 (78B2) Rabbit mAb #2546.

Background

Cyclin-dependent kinase 2 (p33CDK2) is an important component of the cell cycle machinery. Like p34cdc2, kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a CDK inhibitor. Inhibitory phosphorylation occurs on Thr14 and Tyr15 (1). Inhibition of CDK2-cyclin complexes can also be attributed to association with p27 Kip1 and p21 Waf1/Cip1 (2). Activation of CDK2 complexes requires dephosphorylation of Thr14 and Tyr15 by cdc25 phosphatase and phosphorylation of Thr160 (3), which is mediated by CAK, a complex of CDK7 and cyclin H (4). CDK2/cyclin E kinase activity is important for the G1 to S transition and phosphorylation of the Rb protein. During S-phase, active CDK2/cyclin A complexes predominate and phosphorylate E2F and the active CDK2 complex persists in the nucleus throughout G2 (5).

1. Morgan, D.O. (1995) Nature 374, 131-4.
2. Poon, R.Y. et al. (1996) J Biol Chem 271, 13283-91.
3. Gu, Y. et al. (1992) EMBO J 11, 3995-4005.
4. Fesquet, D. et al. (1993) EMBO J 12, 3111-21.
5. Morgan, D.O. (1997) Annu Rev Cell Dev Biol 13, 261-91.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the
antibodies. Protect from light. Do not freeze