PNUTS Antibody

• 产品编号：CST-14171S      品牌：CST       原厂货号：14171S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Specificity / Sensitivity

PNUTS Antibody recognizes endogenous levels of total PNUTS protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val380 of human PNUTS protein. Antibodies are purified by protein A and peptide affinity chromatography.

原厂资料：

Specificity / Sensitivity

PNUTS Antibody recognizes endogenous levels of total PNUTS protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val380 of human PNUTS protein. Antibodies are purified by protein A and peptide affinity chromatography.

Specificity / Sensitivity

PNUTS Antibody recognizes endogenous levels of total PNUTS protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val380 of human PNUTS protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Protein phosphatase-1 nuclear targeting subunit (PNUTS) is one of the key regulators of protein phosphatase 1 (PP1) in the nucleus (1). Via interaction with PP1, PNUTS plays an essential role in multiple cellular processes, including chromatin decondensation (2), DNA damage response (3), and cardiomyocyte apoptosis (4). Notably, PNUTS also regulates the activity of two key tumor suppressors, Rb and p53, through inhibition of PP1 mediated dephosphorylation (5-7). Research studies indicate that PNUTS also sequesters PTEN in the nucleus through direct interaction and inhibits its tumor suppressor function (8). PNUTS is ubiquitously expressed and elevated PNUTS expression is observed in various cancers such as esophageal carcinoma, squamous cell carcinoma, and prostate cancer (1,8).

1. Allen, P.B. et al. (1998) J Biol Chem 273, 4089-95.
2. Landsverk, H.B. et al. (2005) Biochem J 390, 709-17.
3. Landsverk, H.B. et al. (2010) EMBO Rep 11, 868-75.
4. Boon, R.A. et al. (2013) Nature 495, 107-10.
5. Lee, S.J. et al. (2007) Cell Death Differ 14, 1106-16.
6. De Leon, G. et al. (2008) Cancer Biol Ther 7, 833-41.
7. Choy, M.S. et al. (2014) Proc Natl Acad Sci U S A 111, 4097-102.
8. Kavela, S. et al. (2013) Cancer Res 73, 205-14.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not
aliquot the antibody.