Bak (D4E4) Rabbit mAb (PE Conjugate)

• 产品编号：CST-14155S      品牌：CST       原厂货号：14155S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Specificity / Sensitivity

Bak (D4E4) Rabbit mAb (PE Conjugate) recognizes endogenous levels of total Bak protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly75 of human Bak protein.

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Bak (D4E4) Rabbit mAb #12105.

原厂资料：

Specificity / Sensitivity

Bak (D4E4) Rabbit mAb (PE Conjugate) recognizes endogenous levels of total Bak protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly75 of human Bak protein.

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Bak (D4E4) Rabbit mAb #12105.

Background

Bak is a proapoptotic member of the Bcl-2 family (1). This protein is located on the outer membrane of mitochondria and is an essential component for transduction of apoptotic signals through the mitochondrial pathway (2,3). Upon apoptotic stimulation, an upstream stimulator like truncated BID (tBID) induces conformational changes in Bak to form oligomer channels in the mitochondrial membrane for cytochrome c release. The release of cytochrome c to the cytosol activates the caspase-9 pathway and eventually leads to cell death (4,5).

1. Gross, A. et al. (1999) Genes Dev. 13, 1899-1911.
2. Wei, M.C. et al. (2001) Science 292, 727-730.
3. Zong, W.X. et al. (2001) Genes Dev. 15, 1481-1486.
4. Degenhardt, K. et al. (2002) J. Biol. Chem. 277, 14127-14134.
5. Wei, M.C. et al. (2000) Genes Dev. 14, 2060-2071.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the
antibodies. Protect from light. Do not freeze