Mono-Methyl-Histone H3 (Lys36) (D9J1D) Rabbit mAb

• 产品编号：CST-14111S      品牌：CST       原厂货号：14111S
• 产品分类：抗体 > 一抗 > 其它类型一抗
• 应用分类：

描述：

Homology

Species predicted to react based on 100% sequence homology: Hamster, Bovine

Specificity / Sensitivity

Mono-Methyl-Histone H3 (Lys36) (D9J1D) Rabbit mAb recognizes endogenous levels of histone H3 only when mono-methylated at Lys36. The antibody does not cross-react with non-methylated, di-methylated, or tri-methylated Lys36. In addition, the antibody does not cross-react with mono-methylated histone H3 Lys4, Lys9, Lys27, or Lys79.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding mono-methylated Lys36 of human histone H3 protein.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Hamster, Bovine

Specificity / Sensitivity

Mono-Methyl-Histone H3 (Lys36) (D9J1D) Rabbit mAb recognizes endogenous levels of histone H3 only when mono-methylated at Lys36. The antibody does not cross-react with non-methylated, di-methylated, or tri-methylated Lys36. In addition, the antibody does not cross-react with mono-methylated histone H3 Lys4, Lys9, Lys27, or Lys79.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding mono-methylated Lys36 of human histone H3 protein.

Background

The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (1). Histone methylation is a major determinant for the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development (2,3). Arginine methylation of histones H3 (Arg2, 17, 26) and H4 (Arg3) promotes transcriptional activation and is mediated by a family of protein arginine methyltransferases (PRMTs), including the co-activators PRMT1 and CARM1 (PRMT4) (4). In contrast, a more diverse set of histone lysine methyltransferases has been identified, all but one of which contain a conserved catalytic SET domain originally identified in the Drosophila Su(var)3-9, Enhancer of zeste, and Trithorax proteins. Lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing (4). Methylation of these lysine residues coordinates the recruitment of chromatin modifying enzymes containing methyl-lysine binding modules such as chromodomains (HP1, PRC1), PHD fingers (BPTF, ING2), tudor domains (53BP1), and WD-40 domains (WDR5) (5-8). The discovery of histone demethylases such as PADI4, LSD1, JMJD1, JMJD2, and JHDM1 has shown that methylation is a reversible epigenetic marker (9).

1. Peterson, C.L. and Laniel, M.A. (2004) Curr Biol 14, R546-51.
2. Kubicek, S. et al. (2006) Ernst Schering Res Found Workshop , 1-27.
3. Lin, W. and Dent, S.Y. (2006) Curr Opin Genet Dev 16, 137-42.
4. Lee, D.Y. et al. (2005) Endocr Rev 26, 147-70.
5. Daniel, J.A. et al. (2005) Cell Cycle 4, 919-26.
6. Shi, X. et al. (2006) Nature 442, 96-9.
7. Wysocka, J. et al. (2006) Nature 442, 86-90.
8. Wysocka, J. et al. (2005) Cell 121, 859-72.
9. Trojer, P. and Reinberg, D. (2006) Cell 125, 213-7.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody