LC3A/B (D3U4C) XP??Rabbit mAb?(Alexa Fluor??594 Conjugate)

• 产品编号：CST-14079S      品牌：CST       原厂货号：14079S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Homology

Species predicted to react based on 100% sequence homology: Xenopus, Bovine, Dog, Pig

Specificity / Sensitivity

LC3A/B (D3U4C) XP^® Rabbit mAb recognizes endogenous levels of total LC3A and LC3B proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu44 of human LC3B protein (conserved in LC3A).

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor^® 594 fluorescent dye and tested in-house for direct immunofluorescent analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated LC3A/B (D3U4C) XP^® Rabbit mAb #12741.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Xenopus, Bovine, Dog, Pig

Specificity / Sensitivity

LC3A/B (D3U4C) XP^® Rabbit mAb recognizes endogenous levels of total LC3A and LC3B proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu44 of human LC3B protein (conserved in LC3A).

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor^® 594 fluorescent dye and tested in-house for direct immunofluorescent analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated LC3A/B (D3U4C) XP^® Rabbit mAb #12741.

Background

Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation, but it has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection, and cancer (3). Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3) (4) and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy (5). Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows for LC3 to become associated with autophagic vesicles (6-10). The presence of LC3 in autophagosomes and the conversion of LC3 to the lower migrating form, LC3-II, have been used as indicators of autophagy (11).

1. Reggiori, F. and Klionsky, D.J. (2002) Eukaryot. Cell 1, 11-21.
2. Codogno, P. and Meijer, A.J. (2005) Cell Death Differ. 12 Suppl 2, 1509-18.
3. Levine, B. and Yuan, J. (2005) J. Clin. Invest. 115, 2679-88.
4. Mann, S.S. and Hammarback, J.A. (1994) J. Biol. Chem. 269, 11492-97.
5. Lang, T. et al. (1998) EMBO J. 17, 3597-607.
6. Kabeya, Y. et al. (2000) EMBO J. 19, 5720-28.
7. He, H. et al. (2003) J. Biol. Chem. 278, 29278-87.
8. Tanida, I. et al. (2004) J. Biol. Chem. 279, 47704-10.
9. Wu, J. et al. (2006) Biochem. Biophys. Res. Commun. 339, 437-42.
10. Ichimura, Y. et al. (2000) Nature 408, 488-92.
11. Kabeya, Y. et al. (2004) J. Cell Sci. 117, 2805-12.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the
antibody. Protect from light. Do not freeze