Phospho-Doublecortin (Ser334) (D11B10) Rabbit mAb

• 产品编号：CST-14053S      品牌：CST       原厂货号：14053S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Homology

Species predicted to react based on 100% sequence homology: Human

Specificity / Sensitivity

Phospho-Doublecortin (Ser334) (D11B10) Rabbit mAb recognizes endogenous levels of doublecortin protein only when phosphorylated at Ser334. Amino acid Ser334 of mouse doublecortin corresponds to Ser415 of human doublecortin. Proteins of unknown origin may be detected around 90 kDa.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser334 of mouse doublecortin protein.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Human

Specificity / Sensitivity

Phospho-Doublecortin (Ser334) (D11B10) Rabbit mAb recognizes endogenous levels of doublecortin protein only when phosphorylated at Ser334. Amino acid Ser334 of mouse doublecortin corresponds to Ser415 of human doublecortin. Proteins of unknown origin may be detected around 90 kDa.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser334 of mouse doublecortin protein.

Background

Mutations in Doublecortin cause Lissencephaly (smooth brain), a neuronal migration disorder characterized by epilepsy and mental retardation (1). Doublecortin is a microtubule associated protein that stabilizes and bundles microtubules. A conserved doublecortin domain mediates the interaction with microtubules, and interestingly most missense mutations cluster in this domain (2). Kinases JNK, CDK5 and PKA phosphorylate doublecortin. JNK phosphorylates Thr321, Thr331 and Ser334 while PKA phosphorylates Ser47 and CDK5 phosphorylates Ser297 (3-5). Phosphorylation of Ser297 lowers the affinity of doublecortin to microtubules. Furthermore, mutations of Ser297 result in migration defects (5).

Doublecortin phosphorylated at Ser334 is enriched in growth cones and affects neurite outgrowth and neuronal migration (3)

1. Gleeson, J.G. et al. (1998) Cell 92, 63-72.
2. Reiner, O. et al. (2004) Cell Cycle 3, 747-51.
3. Gdalyahu, A. et al. (2004) EMBO J 23, 823-32.
4. Schaar, B.T. et al. (2004) Neuron 41, 203-13.
5. Tanaka, T. et al. (2004) Neuron 41, 215-27.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.