Phospho-PLCγ1 (Tyr783) (D6M9S) Rabbit mAb

• 产品编号：CST-14008S      品牌：CST       原厂货号：14008S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Homology

Species predicted to react based on 100% sequence homology: Rat, Xenopus, Bovine, Dog

Specificity / Sensitivity

Phospho-PLCγ1 (Tyr783) (D6M9S) Rabbit mAb recognizes endogenous levels of PLCγ1 protein only when phosphorylated at Tyr783.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr783 of human PLCγ1 protein.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Rat, Xenopus, Bovine, Dog

Specificity / Sensitivity

Phospho-PLCγ1 (Tyr783) (D6M9S) Rabbit mAb recognizes endogenous levels of PLCγ1 protein only when phosphorylated at Tyr783.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr783 of human PLCγ1 protein.

Background

Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli such as hormones, growth factors, and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP₂) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP₃) and diacylglycerol (DAG) (1). At least four families of PLCs have been identified: PLCβ, PLCγ, PLCδ, and PLCε. Phosphorylation is one of the key mechanisms that regulate the activity of PLC. PLCγ is activated by both receptor and non-receptor tyrosine kinases (2). PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783, and 1245 (3). Phosphorylation by Syk at Tyr783 activates the enzymatic activity of PLCγ1 (4). PLCγ2 is engaged in antigen-dependent signaling in B cells and collagen-dependent signaling in platelets. Phosphorylation by Btk or Lck at Tyr753, 759, 1197, and 1217 is correlated with PLCγ2 activity (5,6).

1. Singer, W.D. et al. (1997) Annu Rev Biochem 66, 475-509.
2. Margolis, B. et al. (1989) Cell 57, 1101-7.
3. Kim, H.K. et al. (1991) Cell 65, 435-41.
4. Wang, Z. et al. (1998) Mol Cell Biol 18, 590-7.
5. Watanabe, D. et al. (2001) J Biol Chem 276, 38595-601.
6. Ozdener, F. et al. (2002) Mol Pharmacol 62, 672-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.