TIA-1 Antibody

• 产品编号：CST-13980S      品牌：Cell Signaling Technology       原厂货号：13980S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Specificity / Sensitivity

TIA-1 Antibody recognizes endogenous levels of total TIA-1 protein. A band of unknown origin is detected at 100 kDa. 

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human TIA-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

原厂资料：

Specificity / Sensitivity

TIA-1 Antibody recognizes endogenous levels of total TIA-1 protein. A band of unknown origin is detected at 100 kDa.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human TIA-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

T-cell intracellular antibody 1 (TIA-1) is a member of the RNA-recognition motif (RRM) family of RNA-binding proteins that was originally found to induce DNA fragmentation in digitonin-permeabilized thymocytes (1). TIA-1 protein has about 80% identity to the related TIAR protein, both of which possess three amino-terminal RRM domains and a glutamine-rich carboxyl terminus (1,2). Alternative splicing is responsible for generating at least two isoforms of TIA-1 and TIAR (3,4). Several research studies indicate that TIA-1 and TIAR play a role in apoptosis, cellular stress, and inflammation. Importantly, TIA-1 and TIAR translocate from the nucleus to stress granules in response to a variety of environmental stresses (5-8). Stress granules function as sites of translational repression in response to potentially damaging conditions. mRNA transcripts targeted by TIA-1 and TIAR include TNF-α, COX-2, cytochrome c, GADD45α, and HIF-1α (8-13).

1. Tian, Q. et al. (1991) Cell 67, 629-39.
2. Kawakami, A. et al. (1992) Proc Natl Acad Sci U S A 89, 8681-5.
3. Izquierdo, J.M. and Valcárcel, J. (2007) J Biol Chem 282, 19410-7.
4. Beck, A.R. et al. (1996) Nucleic Acids Res 24, 3829-35.
5. Kedersha, N.L. et al. (1999) J Cell Biol 147, 1431-42.
6. Gilks, N. et al. (2004) Mol Biol Cell 15, 5383-98.
7. Eisinger-Mathason, T.S. et al. (2008) Mol Cell 31, 722-36.
8. Gottschald, O.R. et al. (2010) J Mol Cell Biol 2, 345-56.
9. Gueydan, C. et al. (1999) J Biol Chem 274, 2322-6.
10. Piecyk, M. et al. (2000) EMBO J 19, 4154-63.
11. Kawai, T. et al. (2006) Mol Cell Biol 26, 3295-307.
12. Dixon, D.A. et al. (2003) J Exp Med 198, 475-81.
13. Lal, A. et al. (2006) Mol Cell 22, 117-28.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not
aliquot the antibody.