Prestained Protein Marker Broad Range (11-190 kDa)

• 产品编号：CST-13953S      品牌：CST       原厂货号：13953S
• 产品分类：蛋白质检测与分析 > 蛋白质凝胶电泳 > 蛋白分子量标准品 > 预染蛋白标准品
• 应用分类：

描述：

Description

Prestained Protein Marker, Broad Range (11-190 kDa) is a mixture of purified proteins covalently coupled to a blue dye that resolve to a series of 11 bands between 11 and 190 kDa following electrophoresis. The protein concentrations are carefully balanced for even intensity. The covalent coupling of the dye to the proteins affects their electrophoretic behavior in SDS-PAGE gels relative to unstained proteins.

原厂资料：

Description

Prestained Protein Marker, Broad Range (11-190 kDa) is a mixture of purified proteins covalently coupled to a blue dye that resolve to a series of 11 bands between 11 and 190 kDa following electrophoresis. The protein concentrations are carefully balanced for even intensity. The covalent coupling of the dye to the proteins affects their electrophoretic behavior in SDS-PAGE gels relative to unstained proteins.

Directions for Use

(a) Transfer the desired amount of the Prestained Protein Marker to a separate tube. For visualizing during electrophoresis, use 5 µl for mini-gels and 10 µl for full-length gels.

(b) Heat the marker to 95-100ºC for 3-5 min.

(c) After a quick microcentrifuge spin, load directly onto a gel. To ensure uniform mobility, load an equal volume of 1X reducing SDS Loading Buffer into any unused wells.

注意事项：

Storage: Supplied in 20 mM Tris-phosphate (pH 7.5 at 25ºC),
3.6 M Urea, 2% (w/v) SDS, 0.2 mM DTT, and 15% glycerol.
Store at -20ºC. Contains approximately 0.2 mg/ml of each
protein.