eRF1 Antibody

• 产品编号：CST-13916S      品牌：CST       原厂货号：13916S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Specificity / Sensitivity

eRF1 Antibody recognizes endogenous levels of total eRF1 protein.

Source / Purification

 Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe117 of human eRF1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

原厂资料：

Specificity / Sensitivity

eRF1 Antibody recognizes endogenous levels of total eRF1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe117 of human eRF1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Efficient termination of mRNA translation in eukaryotes is dependent upon a complex of two polypeptide release factors, eRF1 and eRF3 (1). The eukaryotic translation termination factor 1 (eRF1, ETF1) structurally resembles tRNA, which allows it to participate in stop codon recognition as well as hydrolysis of the peptidyl-tRNA conjugate (2,3). The eRF1 protein contains three functionally distinct domains, including an amino-terminal domain that harbors discrete motifs that participate in stop codon recognition (4,5). Lysine hydroxylation within the amino-terminal domain is required for efficient termination of mRNA translation (6). The central region of eRF1 harbors a GGQ motif that facilitates hydrolysis of peptidyl-tRNA conjugates (7), while its carboxy terminus participates in eRF3 binding (8).

1. Zhouravleva, G. et al. (1995) EMBO J 14, 4065-72.
2. Song, H. et al. (2000) Cell 100, 311-21.
3. Cheng, Z. et al. (2009) Genes Dev 23, 1106-18.
4. Bulygin, K.N. et al. (2010) RNA 16, 1902-14.
5. Frolova, L. et al. (2002) RNA 8, 129-36.
6. Feng, T. et al. (2014) Mol Cell 53, 645-54.
7. Frolova, L.Y. et al. (1999) RNA 5, 1014-20.
8. Merkulova, T.I. et al. (1999) FEBS Lett 443, 41-7.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.