c-Myc (D84C12) Rabbit mAb (Alexa Fluor? 647 Conjugate)

• 产品编号：CST-13871S      品牌：CST       原厂货号：13871S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Homology

Species predicted to react based on 100% sequence homology: Dog, Pig

Specificity / Sensitivity

c-Myc (D84C12) XP^® Rabbit mAb (Alexa Fluor^® 647 Conjugate) detects endogenous levels of total c-Myc protein. This antibody is not recommended for detection of Myc-tagged fusion proteins; for detection of Myc-tagged fusion proteins, use Myc-Tag (9B11) Mouse mAb (Alexa Fluor^® 647 Conjugate) #2233.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues of c-Myc protein.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor^® 647 fluorescent dye and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated c-Myc (D84C12) XP^® Rabbit mAb #5605.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Dog, Pig

Specificity / Sensitivity

c-Myc (D84C12) XP^® Rabbit mAb (Alexa Fluor^® 647 Conjugate) detects endogenous levels of total c-Myc protein. This antibody is not recommended for detection of Myc-tagged fusion proteins; for detection of Myc-tagged fusion proteins, use Myc-Tag (9B11) Mouse mAb (Alexa Fluor^® 647 Conjugate) #2233.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues of c-Myc protein.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor^® 647 fluorescent dye and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated c-Myc (D84C12) XP^® Rabbit mAb #5605.

Background

Members of the Myc/Max/Mad network function as transcriptional regulators with roles in various aspects of cell behavior including proliferation, differentiation and apoptosis (1). These proteins share a common basic-helix-loop-helix leucine zipper (bHLH-ZIP) motif required for dimerization and DNA-binding. Max was originally discovered based on its ability to associate with c-Myc and found to be required for the ability of Myc to bind DNA and activate transcription (2). Subsequently, Max has been viewed as a central component of the transcriptional network, forming homodimers as well as heterodimers with other members of the Myc and Mad families (1). The association between Max and either Myc or Mad can have opposing effects on transcriptional regulation and cell behavior (1). The Mad family consists of four related proteins; Mad1, Mad2 (Mxi1), Mad3 and Mad4, and the more distantly related members of the bHLH-ZIP family, Mnt and Mga. Like Myc, the Mad proteins are tightly regulated with short half-lives. In general, Mad family members interfere with Myc-mediated processes such as proliferation, transformation and prevention of apoptosis by inhibiting transcription (3,4).

1. Baudino, T.A. and Cleveland, J.L. (2001) Mol Cell Biol 21, 691-702.
2. Blackwood, E.M. and Eisenman, R.N. (1991) Science 251, 1211-7.
3. Henriksson, M. and Lüscher, B. (1996) Adv Cancer Res 68, 109-82.
4. Grandori, C. et al. (2000) Annu Rev Cell Dev Biol 16, 653-99.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the
antibody. Protect from light. Do not freeze