LARS Antibody

• 产品编号：CST-13868S      品牌：CST       原厂货号：13868S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Specificity / Sensitivity

LARS Antibody recognizes endogenous levels of total LARS protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human LARS protein. Antibodies are purified by protein A and peptide affinity chromatography.

原厂资料：

Specificity / Sensitivity

LARS Antibody recognizes endogenous levels of total LARS protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human LARS protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Leucyl-tRNA Synthetase (LARS) is a leucine sensor critical for the activation of mTORC1 (1). mTORC1 kinase complex is an important component in the regulation of cell growth (2,3). Its activity is modulated by energy levels, growth factors, and amino acids (4,5). The four related GTPases, RagA, RagB, RagC, and RagD, have been shown to interact with raptor in mTORC1 (2,3). These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals (2,3). LARS functions as a GTPase-activating protein (GAP) and interacts directly with RagD GTPase (1). The role of LARS in leucine sensing is not related to its tRNA charging activity (1).

1. Han, J.M. et al. (2012) Cell 149, 410-24.
2. Sancak, Y. et al. (2008) Science 320, 1496-501.
3. Kim, E. et al. (2008) Nat Cell Biol 10, 935-45.
4. Hay, N. and Sonenberg, N. (2004) Genes Dev 18, 1926-45.
5. Wullschleger, S. et al. (2006) Cell 124, 471-84.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not
aliquot the antibody.