VAMP3 Antibody

• 产品编号：CST-13640S      品牌：CST       原厂货号：13640S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Specificity / Sensitivity

VAMP3 recognizes endogenous levels of total VAMP3 protein. This antibody does not cross-react with VAMP1 or VAMP2 proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human VAMP3 protein. Antibodies are purified by protein A and peptide affinity chromatography.

原厂资料：

Specificity / Sensitivity

VAMP3 recognizes endogenous levels of total VAMP3 protein. This antibody does not cross-react with VAMP1 or VAMP2 proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human VAMP3 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Proteins in the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex are integral membrane proteins involved in vesicle transport and membrane fusion by pairing of vesicular SNAREs (v-SNAREs) with cognate target SNAREs (t-SNAREs) (reviewed in 1,2). Vesicle associated membrane protein 3 (VAMP3), also known as cellubrevin, has a broad tissue distribution and localizes to endosomal compartments (3). VAMP3 interacts with the t-SNAREs syntaxin1, syntaxin4, SNAP23, and SNAP25 (4,5). Research studies indicate that VAMP3 is involved in transferrin receptor recycling to the plasma membrane (6) and in T-cell receptor recycling to immunological synapses (7). Inhibition of VAMP3 with tetanus toxin impairs membrane trafficking during cell migration (8).

1. Jena, B.P. (2011) Adv Exp Med Biol 713, 13-32.
2. Kasai, H. et al. (2012) Physiol Rev 92, 1915-64.
3. McMahon, H.T. et al. (1993) Nature 364, 346-9.
4. Chilcote, T.J. et al. (1995) J Cell Biol 129, 219-31.
5. Schraw, T.D. et al. (2003) Biochem J 374, 207-17.
6. Galli, T. et al. (1994) J Cell Biol 125, 1015-24.
7. Das, V. et al. (2004) Immunity 20, 577-88.
8. Tayeb, M.A. et al. (2005) Exp Cell Res 305, 63-73.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.