PHC1 Antibody

• 产品编号：CST-13505S      品牌：CST       原厂货号：13505S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PHC1 Antibody 能够检测内源性的PHC1总蛋白。该多克隆抗体是采用合成的与人源PHC1蛋白近羧基端氨基酸肽段免疫动物而生产的。由蛋白质A和多肽亲和层析纯化而成。polycomb group (PcG) 蛋白通过维持细胞谱系特化，细胞死亡和细胞周期抑制以促进维持细胞身份，干细胞再生，细胞周期调控以及肿瘤发生（1-4）。Polycomb group protein可以抑制p16Ink4a和 p19Arf基因，从而调控细胞增殖以及衰老。同时他们对于维持成年造血，新生干细胞和胚胎干细胞是至关重要的（3-5）。PcG蛋白在两种复合体中被发现，这两种复合体协同作用以维持通过染色质的表观遗传修饰而长期基因沉默。DNA结合转录因子将EED-EZH2招募到基因上，从而使组蛋白H3的Lys27位点发生甲基化（6）。Lys27位点的甲基化有助于PRC1复合体的募集，从而将组蛋白H2A Lys119位点泛素化（7）。PRC1是有BMI1和RING1A组成，能够增强RING1B催化亚基的E3泛素连接酶活性（8）。 Polyhomeotic-like 1 (PHC1)是几个另外的PRC1复合体蛋白中的一个，是维持PRC1靶基因沉默状态以及介导发育过程中正确的前后方向特化所必需的（9）。PHC1基因上相应的突变与先天性小头畸形的常染色体隐性形式有关，该病的特征是认知功能低于正常人并且DNA修复受损（10）。

1. Boyer, L.A. et al. (2006) Nature 441, 349-53.
2. Lee, T.I. et al. (2006) Cell 125, 301-13.
3. Park, I.K. et al. (2003) Nature 423, 302-5.
4. Molofsky, A.V. et al. (2003) Nature 425, 962-7.
5. Molofsky, A.V. et al. (2005) Genes Dev 19, 1432-7.
6. Cao, R. and Zhang, Y. (2004) Mol Cell 15, 57-67.
7. Wang, H. et al. (2004) Nature 431, 873-8.
8. Cao, R. et al. (2005) Mol Cell 20, 845-54.
9. Isono, K. et al. (2005) Mol Cell Biol 25, 6694-706.
10. Awad, S. et al. (2013) Hum Mol Genet 22, 2200-13.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Rat, Bovine

Specificity / Sensitivity

PHC1 Antibody recognizes endogenous levels of total PHC1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PHC1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The polycomb group (PcG) proteins contribute to the maintenance of cell identity, stem cell self-renewal, cell cycle regulation, and oncogenesis by maintaining the silenced state of genes that promote cell lineage specification, cell death, and cell-cycle arrest (1-4). Polycomb group proteins regulate cell proliferation and senescence through repression of the p16Ink4a and p19Arf genes, and are essential in maintaining adult hematopoietic, neural stem cells, and embryonic stem cells (3-5). PcG proteins are found in two complexes that cooperate to maintain long-term gene silencing through epigenetic chromatin modifications. DNA-binding transcription factors recruit the EED-EZH2 complex to genes, which methylates histone H3 on Lys27 (6). Methylation of Lys27 facilitates the recruitment of the PRC1 complex, which ubiquitinylates histone H2A on Lys119 (7). PRC1 is composed of BMI1 and RING1A, which enhance the E3 ubiquitin ligase activity of the RING1B catalytic subunit (8). Polyhomeotic-like 1 (PHC1) is one of several additional PRC1 complex proteins that are required to maintain the silenced state of PRC1 target genes and mediate proper anterior-posterior specification during development (9). Mutations in the corresponding PHC1 gene correlate with an autosomal recessive form of primary microcephaly characterized by low-to-normal cognitive function and impaired DNA repair (10).

1. Boyer, L.A. et al. (2006) Nature 441, 349-53.
2. Lee, T.I. et al. (2006) Cell 125, 301-13.
3. Park, I.K. et al. (2003) Nature 423, 302-5.
4. Molofsky, A.V. et al. (2003) Nature 425, 962-7.
5. Molofsky, A.V. et al. (2005) Genes Dev 19, 1432-7.
6. Cao, R. and Zhang, Y. (2004) Mol Cell 15, 57-67.
7. Wang, H. et al. (2004) Nature 431, 873-8.
8. Cao, R. et al. (2005) Mol Cell 20, 845-54.
9. Isono, K. et al. (2005) Mol Cell Biol 25, 6694-706.
10. Awad, S. et al. (2013) Hum Mol Genet 22, 2200-13.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody