PACT (D9N6J) Rabbit mAb

• 产品编号：CST-13490S      品牌：CST       原厂货号：13490S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PACT (D9N6J) Rabbit mAb能够检测内源性的PACT总蛋白。该单克隆抗体是用合成的对应于人PACT蛋白的近氨基末端残基序列的多肽免疫动物而生产的。PACT (protein activator of protein kinase R)是一种双链RNA结合蛋白和PKR (protein kinase R)的细胞激活因子。PKR是一种能够介导干扰素抗病毒和抗增殖作用的一种激酶（1）。压力信号血清饥饿和亚砷酸盐处理可使PACT与PKR形成异二聚体，从而导致eIF-2α的磷酸化以及蛋白合成的抑制（1,20）。PACT还可以刺激Dicer的活性在RNA介导的基因剪接中发挥作用，从而影响miRNA累积以及siRNA基因剪接的效率（3,4）。最近的研究表明，PACT还可以与病毒核酸传感蛋白RIG-I相互作用，并且刺激RIG-I-induced type I干扰素的产生（5）。研究者发现PACT突变与肌张力障碍有关，肌张力障碍是一种运动障碍，病人会呈现不随意肌收缩和姿态（6-8）。

1. Patel, R.C. and Sen, G.C. (1998) EMBO J 17, 4379-90.
2. Patel, C.V. et al. (2000) J Biol Chem 275, 37993-8.
3. Lee, Y. et al. (2006) EMBO J 25, 522-32.
4. Koscianska, E. et al. (2011) PLoS One 6, e28548.
5. Kok, K.H. et al. (2011) Cell Host Microbe 9, 299-309.
6. Bragg, D.C. et al. (2011) Neurobiol Dis 42, 136-47.
7. Camargos, S. et al. (2008) Lancet Neurol 7, 207-15.
8. Seibler, P. et al. (2008) Lancet Neurol 7, 380-1.

原厂资料：

Specificity / Sensitivity

PACT (D9N6J) Rabbit mAb recognizes endogenous levels of total PACT protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PACT protein.

Background

PACT (protein activator of protein kinase R) is a double stranded RNA binding protein and a cellular activator of PKR (protein kinase R), a kinase that mediates the antiviral and antiproliferative actions of interferon (1). Stress signals such as serum starvation and arsenite treatment induces PACT to heterodimerize with PKR, resulting in the phosphorylation of eIF-2α and inhibition of protein synthesis (1,2). PACT has also been shown to play a role in RNA-mediated gene silencing by stimulating the activity of Dicer, thereby affecting the efficiency of miRNA accumulation and siRNA gene silencing (3,4). More recently, PACT has been shown to interact with RIG-I, a sensor of viral nucleic acids, and stimulates RIG-I-induced type I interferon production (5). Researchers have found that mutations in PACT are associated with dystonia, a movement disorder where patients develop involuntary muscle contractions and postures (6-8).

1. Patel, R.C. and Sen, G.C. (1998) EMBO J 17, 4379-90.
2. Patel, C.V. et al. (2000) J Biol Chem 275, 37993-8.
3. Lee, Y. et al. (2006) EMBO J 25, 522-32.
4. Koscianska, E. et al. (2011) PLoS One 6, e28548.
5. Kok, K.H. et al. (2011) Cell Host Microbe 9, 299-309.
6. Bragg, D.C. et al. (2011) Neurobiol Dis 42, 136-47.
7. Camargos, S. et al. (2008) Lancet Neurol 7, 207-15.
8. Seibler, P. et al. (2008) Lancet Neurol 7, 380-1.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.