PathScan??Total Mer Sandwich ELISA Kit

• 产品编号：CST-13488C      品牌：Cell Signaling Technology       原厂货号：13488C
• 产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测试剂盒
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描述：

Specificity / Sensitivity

PathScan^® Total Mer Sandwich ELISA Kit recognizes endogenous levels of Mer protein in human cells, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Description

PathScan^® Total Mer Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Mer protein. A Mer Rabbit mAb has been coated onto the microwells. After incubation with cell lysates, Mer protein (phospho and nonphospho) is captured by the coated antibody. Following extensive washing, a Mer Mouse Detection mAb is added to detect the captured Mer proteins. Anti-mouse IgG, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of optical density for this developed color is proportional to the quantity of Mer protein.

原厂资料：

Specificity / Sensitivity

PathScan^® Total Mer Sandwich ELISA Kit recognizes endogenous levels of Mer protein in human cells, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Description

PathScan^® Total Mer Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Mer protein. A Mer Rabbit mAb has been coated onto the microwells. After incubation with cell lysates, Mer protein (phospho and nonphospho) is captured by the coated antibody. Following extensive washing, a Mer Mouse Detection mAb is added to detect the captured Mer proteins. Anti-mouse IgG, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of optical density for this developed color is proportional to the quantity of Mer protein.

Background

Mer tyrosine kinase belongs to a receptor tyrosine kinase family with Axl and Tyro3. This family is characterized by a common NCAM (neural adhesion molecule)-related extracellular domain and a common ligand, GAS6 (growth arrest-specific protein 6). Mer protein has an apparent molecular weight of 170-210 kDa due to different glycosylation patterns generated in different cell types. Mer can be activated by dimerization and autophosphorylation through ligand binding or homophilic cell-cell interaction mediated by its NCAM-like motif (1). The downstream signaling components of activated Mer include PI3 kinase, PLCγ, and MAP kinase (2). Family members are prone to transcriptional regulation and carry out diverse functions including the regulation of cell adhesion, migration, phagocytosis, and survival (3). Mer regulates macrophage activation, promotes apoptotic cell engulfment, and supports platelet aggregation and clot stability in vivo (4). Investigators have found that overexpression of Mer may play a cooperative role in leukemogenesis and may be an effective target for biologically based leukemia/lymphoma therapy (5).

1. Ling, L. et al. (1996) J Biol Chem 271, 18355-62.
2. Ling, L. and Kung, H.J. (1995) Mol Cell Biol 15, 6582-92.
3. Hafizi, S. and Dahlbäck, B. (2006) Cytokine Growth Factor Rev 17, 295-304.
4. Sather, S. et al. (2007) Blood 109, 1026-33.
5. Keating, A.K. et al. (2006) Oncogene 25, 6092-100.

注意事项：

* 12 8-well modules -Each module is designed to break apart for 8 tests.
**Kit should be stored at 4°C with the exception of 10X Cell Lysis Buffer, which is stored at –20°C (packaged separately).