CHD1L (E1I8C) Rabbit mAb

• 产品编号：CST-13460S      品牌：CST       原厂货号：13460S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CHD1L (E1I8C) Rabbit mAb兔单抗能够检测内源性CHD1L总蛋白水平。该单克隆抗体是采用合成的与人源CHD1L蛋白的Gly231周围序列相对应的肽段免疫动物而生产的。(CHD1L)是一种DNA解旋酶，是ATP依赖的染色质重构酶SNF2亚家族的成员(1)。 与其他CHD染色质重构蛋白不同，CHD1L缺乏一种甲基化组蛋白结合染色质域，但是却含有一个macro 结构域能够的poly-ADP ribosylated (PARylated)靶标结合（1,2）。在基因毒性压力后，CHD1L与PARylated PARP1相互作用，并被招募到DNA损伤位点以有助于DNA修复（3,4）。CHD1L蛋白在metastatic hepatocellular carcinoma (HCC)中常被过量表达，并且对应的基因在HCC中定位在一个被频繁扩增的区域内（5-7）。研究表明 CHD1L过表达可能会导致染色质的过度松弛并将下面的DNA暴露于基因毒性压力中（1）。 CHD1L可以调控促进肿瘤细胞增殖迁移以及转移的基因的表达，同时提供了另外一种机制即 CHD1L可能会促进肝细胞癌的发展和转移（6,8）。 另外的研究表明膀胱癌，结肠癌以及卵巢癌的发展中都可能涉及到CHD1L的过表达(9-11)。

1. Deng, W. (2009) Cell Stem Cell 5, 349-50.
2. Karras, G.I. et al. (2005) EMBO J 24, 1911-20.
3. Gottschalk, A.J. et al. (2009) Proc Natl Acad Sci U S A 106, 13770-4.
4. Ahel, D. et al. (2009) Science 325, 1240-3.
5. Ma, N.F. et al. (2008) Hepatology 47, 503-10.
6. Chen, L. et al. (2010) J Clin Invest 120, 1178-91.
7. Chen, L. et al. (2009) Hepatology 50, 122-9.
8. Chen, M. et al. (2009) PLoS One 4, e6727.
9. He, W.P. et al. (2012) BMC Cancer 12, 437.
10. Ji, X. et al. (2013) J Surg Res 185, 84-91.
11. Tian, F. et al. (2013) Tumour Biol , [Epub ahead of print].

原厂资料：

Specificity / Sensitivity

CHD1L (E1I8C) Rabbit mAb recognizes endogenous levels of total CHD1L protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly231 of human CHD1L protein.

Background

Chromodomain-helicase-DNA-binding domain 1 like (CHD1L) is a DNA helicase and member of the SNF2 subfamily of ATP dependent chromatin remodeling enzymes (1). Unlike other CHD chromatin remodeling proteins, CHD1L lacks a methylated histone binding chromodomain but does contain a macro domain that binds poly-ADP ribosylated (PARylated) targets (1,2). Following genotoxic stress, CHD1L interacts with PARylated PARP1 and is recruited to sites of DNA damage to facilitate DNA repair (3,4). The CHD1L protein is often over expressed in metastatic hepatocellular carcinoma (HCC) and the corresponding CHD1L gene is located in a region that is frequently amplified in cases of HCC (5-7). Research studies indicate that CHD1L over expression may lead to over relaxation of chromatin and exposing the underlying DNA to genotoxic stress (1). CHD1L can regulate expression of genes that promote tumor cell proliferation, migration and metastasis, providing another mechanism where CHD1L may promote hepatocellular carcinoma progression and metastasis (6,8). Additional research studies suggest that over expression of CHD1L may be involved in the progression of bladder, colon and ovary cancer (9-11). Chromodomain-helicase-DNA-binding domain 1 like.

1. Deng, W. (2009) Cell Stem Cell 5, 349-50.
2. Karras, G.I. et al. (2005) EMBO J 24, 1911-20.
3. Gottschalk, A.J. et al. (2009) Proc Natl Acad Sci U S A 106, 13770-4.
4. Ahel, D. et al. (2009) Science 325, 1240-3.
5. Ma, N.F. et al. (2008) Hepatology 47, 503-10.
6. Chen, L. et al. (2010) J Clin Invest 120, 1178-91.
7. Chen, L. et al. (2009) Hepatology 50, 122-9.
8. Chen, M. et al. (2009) PLoS One 4, e6727.
9. He, W.P. et al. (2012) BMC Cancer 12, 437.
10. Ji, X. et al. (2013) J Surg Res 185, 84-91.
11. Tian, F. et al. (2013) Tumour Biol , [Epub ahead of print].

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody