EXPAND1/MUM1 (D1Z6Y) Rabbit mAb

• 产品编号：CST-13437S      品牌：CST       原厂货号：13437S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

EXPAND1/MUM1 (D1Z6Y) Rabbit mAb 能识别内源性总的EXPAND1/MUM1 蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与人EXPAND1/MUM1蛋白Arg692附近氨基酸一致。DNA双链损伤（DSBs）是潜在的有害损伤区域可能由电离辐射（IR），拟辐射化合物，或DNA复制抑制因子所引发。黑色素瘤相关抗原（mutated）1（MUM1,EXPAND1）是一个含有PWWP-结构域的染色质结合蛋白，涉及了维持分裂中期染色体的染色质结构。当响应DNA损伤后，EXPAND1/MUM1通过直接和DNA损伤因子53BP1结合募集到DNA双链损伤区（1）。EXPAND1/MUM1募集到DNA损伤区被认为会修饰染色质的结构使其与其他DNA修复因子结合（2）。53BP1激发了检验点激酶ATM活性，促进DNA双链通过非同源末端连接（NHEJ）修复途径修复损伤（3）。

1. Huen, M.S. et al. (2010) Mol Cell 37, 854-64.
2. Sy, S.M. et al. (2010) Nucleus 1, 472-4.
3. Mochan, T.A. et al. (2004) DNA Repair (Amst) 3, 945-52.

原厂资料：

Specificity / Sensitivity

EXPAND1/MUM1 (D1Z6Y) Rabbit mAb recognizes endogenous levels of total EXPAND1/MUM1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg692 of human EXPAND1/MUM1 protein.

Background

DNA double-strand breaks (DSBs) are potentially hazardous lesions that can be induced by ionizing radiation (IR), radiomimetic chemicals, or DNA replication inhibitors. Melanoma associated antigen (mutated) 1 (MUM1, EXPAND1) is a PWWP-domain containing chromatin binding protein involved in maintaining chromatin architecture of interphase chromosomes. In response to DNA damage, EXPAND1/MUM1 accumulates at sites of DNA double strand breaks through direct interaction with DNA repair factor 53BP1 (1). Accumulation of EXPAND1/MUM1 at damaged DNA sites is thought to modify the structure of the chromatin and allow access to other DNA repair factors (2). 53BP1 activates the checkpoint kinase ATM and promotes DNA double strand break repair via nonhomologous end joining (NHEJ) repair pathway (3).

1. Huen, M.S. et al. (2010) Mol Cell 37, 854-64.
2. Sy, S.M. et al. (2010) Nucleus 1, 472-4.
3. Mochan, T.A. et al. (2004) DNA Repair (Amst) 3, 945-52.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.