NRMT (D9D6P) Rabbit mAb

• 产品编号：CST-13432S      品牌：CST       原厂货号：13432S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

NRMT (D9D6P) Rabbit mAb可以识别内源性NRMT总蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与人NRMT蛋白Leu49附近氨基酸一致。N末端RCC1甲基转移酶（NRMT），最初被认为是甲基转移酶样蛋白11A(METTL11A),是甲基转移酶11家族蛋白的一员，也是人体最早被发现的α-N-甲基转移酶（1-3）。free α-amino家族的氨基端甲基转移是一个翻译后修饰过程，其间Met残基被脱离，然后暴露的α-amino家族被NRMT单甲基化，双甲基化或多甲基化（4）。NRMT甲基化含有氨基端Met-X-Pro-Lys基序的蛋白，其中X可以是丙氨酸、脯氨酸、或丝氨酸残基(4)。NRMT的底物包括Ran 鸟嘌呤核苷酸转移因子(RCC1), SET/TAF-1/PHAP-II, 成视网膜细胞瘤(Rb),和 CENP-B (3-6). RCC1的α-N-甲基化对于与染色质的结合，正常双极纺锤体形成和染色质分离都是必须的（3-5）。CENP-B的α-N-甲基化也能调控CENP-B和着丝粒DNA的结合（6）。

1. Wrighton, K.H. (2010) Nat Rev Mol Cell Biol 11, 604-5.
2. Webb, K.J. et al. (2010) Biochemistry 49, 5225-35.
3. Tooley, C.E. et al. (2010) Nature 466, 1125-8.
4. Petkowski, J.J. et al. (2012) Biochemistry 51, 5942-50.
5. Chen, T. et al. (2007) Nat Cell Biol 9, 596-603.
6. Dai, X. et al. (2013) J Proteome Res 12, 4167-75.

原厂资料：

Specificity / Sensitivity

NRMT (D9D6P) Rabbit mAb recognizes endogenous levels of total NRMT protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu49 of human NRMT protein.

Background

N-terminal RCC1 methyltransferase (NRMT), formerly known as methyltransferase-like protein 11A (METTL11A), is a member of the methyltransferase 11 family of proteins and is the first α-N-methyltransferase to be discovered in humans (1-3). Amino-terminal methylation of free α-amino groups is a post-translational modification where an initiating Met residue is cleaved and the exposed α–amino group is mono-, di-, or trimethylated by NRMT (4). NRMT methylates proteins containing an amino-terminal Met-X-Pro-Lys motif, where X is an alanine, proline, or serine residue (4). Substrates of NRMT include the Ran guanine nucleotide-exchange factor (RCC1), SET/TAF-1/PHAP-II, retinoblastoma (Rb), and CENP-B (3-6). α-N-methylation of RCC1 is required for efficient binding to chromatin, securing normal bipolar spindle formation and chromosome segregation (3,5). α-N-methylation of CENP-B also appears to regulate CENP-B binding to centromeric DNA (6).

1. Wrighton, K.H. (2010) Nat Rev Mol Cell Biol 11, 604-5.
2. Webb, K.J. et al. (2010) Biochemistry 49, 5225-35.
3. Tooley, C.E. et al. (2010) Nature 466, 1125-8.
4. Petkowski, J.J. et al. (2012) Biochemistry 51, 5942-50.
5. Chen, T. et al. (2007) Nat Cell Biol 9, 596-603.
6. Dai, X. et al. (2013) J Proteome Res 12, 4167-75.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.