CNOT6 (E1L8F) Rabbit mAb

• 产品编号：CST-13415S      品牌：CST       原厂货号：13415S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CNOT6 (E1L8F) Rabbit mAb能够检测内源性的CNOT6总蛋白水平。该单克隆抗体是用围绕人CNOT6 蛋白Gly400位点的氨基酸序列合成的多肽免疫动物而生产的。进化保守的CCR4-NOT (CNOT) 复合物能够调控真核细胞中的mRNA代谢（1）。这种调控作用发生在mRNA合成和降解的不同水平，包括转录起始，延伸，去乙酰化以及降解（1）。已经鉴定出了该复合物中存在的多种组分包括CNOT1, CNOT2, CNOT3, CNOT4, CNOT6, CNOT7, CNOT8, CNOT9, 以及CNOT10（2）。另外，该复合物的亚基组成在不同的组织中是各异的（3）。不同的CNOT蛋白可与CNOT6, CNOT6L, CNOT7 和CNOT8一起呈现出3'-5' RNAse活性，执行不同的功能（2,4）。研究表明，CCR4-NOT deadenylase 亚基CNOT6 和 CNOT6L能够介导细胞存活并在细胞增殖，P-body形成以及基因表达调控中具有重要作用（5）。此外还有研究证据表明，CNOT6能够作为核激素受体共激活因子与ZNF335 (NIF-1)一起调控特定的RARα 调控的基因的表达（6）.

1. Denis, C.L. and Chen, J. (2003) Prog Nucleic Acid Res Mol Biol 73, 221-50.
2. Lau, N.C. et al. (2009) Biochem J 422, 443-53.
3. Chen, C. et al. (2011) Biochem Biophys Res Commun 411, 360-4.
4. Chen, J. et al. (2002) EMBO J 21, 1414-26.
5. Mittal, S. et al. (2011) Mol Biol Cell 22, 748-58.
6. Garapaty, S. et al. (2008) J Biol Chem 283, 6806-16.

原厂资料：

Specificity / Sensitivity

CNOT6 (E1L8F) Rabbit mAb recognizes endogenous levels of total CNOT6 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly400 of human CNOT6 protein.

Background

The evolutionarily conserved CCR4-NOT (CNOT) complex regulates mRNA metabolism in eukaryotic cells (1). This regulation occurs at different levels of mRNA synthesis and degradation, including transcription initiation, elongation, deadenylation, and degradation (1). Multiple components, including CNOT1, CNOT2, CNOT3, CNOT4, CNOT6, CNOT6L, CNOT7, CNOT8, CNOT9, and CNOT10 have been identified in this complex (2). In addition, subunit composition of this complex has been shown to vary among different tissues (3).The various CNOT proteins display different functions, with CNOT6, CNOT6L, CNOT7 and CNOT8 exhibiting 3'-5' RNAse activity (2,4). Research studies indicate that the CCR4-NOT deadenylase subunits CNOT6 and CNOT6L help mediate cell survival and play a role in cell proliferation, P-body formation, and regulation of gene expression (5). Additional research evidence suggests that CNOT6 can act as a nuclear hormone receptor coactivator that associates with ZNF335 (NIF-1) to regulate expression from specific RARα regulated genes (6).

1. Denis, C.L. and Chen, J. (2003) Prog Nucleic Acid Res Mol Biol 73, 221-50.
2. Lau, N.C. et al. (2009) Biochem J 422, 443-53.
3. Chen, C. et al. (2011) Biochem Biophys Res Commun 411, 360-4.
4. Chen, J. et al. (2002) EMBO J 21, 1414-26.
5. Mittal, S. et al. (2011) Mol Biol Cell 22, 748-58.
6. Garapaty, S. et al. (2008) J Biol Chem 283, 6806-16.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.