Enolase-1 (D2S1A) Rabbit mAb

• 产品编号：CST-13410S      品牌：CST       原厂货号：13410S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Enolase-1 (D2S1A) Rabbit mAb兔单抗可以识别内源性enolase-1总蛋白水平。单抗由合成肽段免疫动物获得，抗体和人enolase-1蛋白的Leu171邻近氨基酸序列一致。烯醇酶是糖酵解过程的的关键酶之一，负责将2－磷酸甘油酸催化成磷酸烯醇式丙酮酸。哺乳动物的烯醇酶有三个亚基：烯醇酶1（α－烯醇酶），烯醇酶2（γ－烯醇酶）和烯醇酶3（β－烯醇酶），它们可以形成同源或异源二聚体。不同的组织表达不同的同工酶形式（1）。烯醇酶1在低氧条件下的无氧代谢中发挥关键作用，它可以在组织侵袭中作为一个细胞表面纤溶酶原的受体（2,3）。在一些乳腺癌和肺癌和肿瘤进展过程中发现烯醇酶1的异常表达（4－7）。相对的，一种烯醇酶1的剪切突变形式（MBP－1）和C－myc的启动子p2结合，发挥肿瘤抑制因子的作用。正是由于这个原因，在一些肿瘤的治疗中，enolase-1被认为是一个潜在的治疗靶点（8）。

1. Pancholi, V. (2001) Cell Mol Life Sci 58, 902-20.
2. Redlitz, A. et al. (1995) Eur J Biochem 227, 407-15.
3. Jiang, B.H. et al. (1997) Cancer Res 57, 5328-35.
4. Peebles, K.A. et al. (2003) Carcinogenesis 24, 651-7.
5. Zhang, L. et al. (2000) J Surg Res 93, 108-19.
6. Wu, W. et al. (2002) Clin Exp Metastasis 19, 319-26.
7. Hennipman, A. et al. (1988) Tumour Biol 9, 241-8.
8. Feo, S. et al. (2000) FEBS Lett 473, 47-52.

原厂资料：

Specificity / Sensitivity

Enolase-1 (D2S1A) Rabbit mAb recognizes endogenous levels of total enolase-1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu171 of human enolase-1 protein.

Background

Enolase is an important glycolytic enzyme involved in the interconversion of 2-phosphoglycerate to phosphoenolpyruvate. Mammalian enolase exists as three subunits: enolase-1 (α-enolase), enolase-2 (γ-enolase) and enolase-3 (β-enolase) that can form both homo- and heterodimers. Expression of the enolase isoforms differs in a tissue specific manner (1). Enolase-1 plays a key role in anaerobic metabolism under hypoxic conditions and may act as a cell surface plasminogen receptor during tissue invasion (2,3). Abnormal expression of enolase-1 is associated with tumor progression in some cases of breast and lung cancer (4-7). Alternatively, an enolase-1 splice variant (MBP-1) binds the c-myc promoter p2 and may function as a tumor suppressor. For this reason enolase-1 is considered as a potential therapeutic target in the treatment of some forms of cancer (8).

1. Pancholi, V. (2001) Cell Mol Life Sci 58, 902-20.
2. Redlitz, A. et al. (1995) Eur J Biochem 227, 407-15.
3. Jiang, B.H. et al. (1997) Cancer Res 57, 5328-35.
4. Peebles, K.A. et al. (2003) Carcinogenesis 24, 651-7.
5. Zhang, L. et al. (2000) J Surg Res 93, 108-19.
6. Wu, W. et al. (2002) Clin Exp Metastasis 19, 319-26.
7. Hennipman, A. et al. (1988) Tumour Biol 9, 241-8.
8. Feo, S. et al. (2000) FEBS Lett 473, 47-52.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody