Phospho-Rad17 (Ser635) (D6K9P) Rabbit mAb

• 产品编号：CST-13404S      品牌：CST       原厂货号：13404S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Rad17 (Ser635) (D6K9P) Rabbit mAb可以识别Ser635被磷酸化的内源性Rad17.单克隆抗体由合成肽段免疫动物产生，该肽段与人Rad17的Ser635邻近残基序列一致。人检验点蛋白Rad17和它的裂变和出芽酵母同源物（分别是Schizosaccharomyces pombeRad17和Saccharomyces cerevisiae Rad24）涉及到DNA损伤和DNA合成中止引起的检验点信号激活（1-4）。使用基因毒性药物处理人细胞会诱导ATM/ATR依赖的Rad17的Ser635和Ser645磷酸化。Rad17磷酸化是DNA损伤细胞检验点信号早期的重要事件（5-7）。

1. Griffiths, D.J. et al. (1995) EMBO J 14, 5812-23.
2. Li, L. et al. (1999) Oncogene 18, 1689-99.
3. Bao, S. et al. (1998) Cell Growth Differ 9, 961-7.
4. von Deimling, F. et al. (1999) Hum Genet 105, 17-27.
5. Bao, S. et al. (2001) Nature 411, 969-74.
6. Post, S. et al. (2001) Proc Natl Acad Sci U S A 98, 13102-7.
7. Wang, X. et al. (2001) Cancer Res 61, 7417-21.

原厂资料：

Specificity / Sensitivity

Phospho-Rad17 (Ser635) (D6K9P) Rabbit mAb recognizes endogenous levels of Rad17 protein only when phosphorylated at Ser635.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser635 of human Rad17 protein.

Background

The human checkpoint protein Rad17 and its fission and budding yeast orthologues (Schizosaccharomyces pombeRad17 and Saccharomyces cerevisiae Rad24, respectively) are involved in the activation of checkpoint signals in response to DNA damage or disruption of DNA synthesis (1-4). Treatment of human cells with genotoxic agents induces ATM/ATR-dependent phosphorylation of Rad17 at Ser635 and Ser645. Rad17 phosphorylation is a critical early event during checkpoint signaling in DNA-damaged cells (5-7).

1. Griffiths, D.J. et al. (1995) EMBO J 14, 5812-23.
2. Li, L. et al. (1999) Oncogene 18, 1689-99.
3. Bao, S. et al. (1998) Cell Growth Differ 9, 961-7.
4. von Deimling, F. et al. (1999) Hum Genet 105, 17-27.
5. Bao, S. et al. (2001) Nature 411, 969-74.
6. Post, S. et al. (2001) Proc Natl Acad Sci U S A 98, 13102-7.
7. Wang, X. et al. (2001) Cancer Res 61, 7417-21.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody