Nogo-A Antibody

• 产品编号：CST-13401S      品牌：CST       原厂货号：13401S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Nogo-A Antibody 兔多抗可以识别内源性的Nogo-A总蛋白。基于序列同源性，该抗体不能识别Nogo-B或 Nogo-C。该多克隆抗体由合成的人Nogo-A蛋白Gly220邻近肽段免疫动物而获得。抗体通过蛋白A和多肽亲和层析纯化。神经突增生抑制蛋白(Nogo, RTN4)被鉴定为中枢神经系统(CNS)的轴突生长抑制剂，属于浆膜蛋白家族。Nogo有三种主要的异构体(Nogo-A, Nogo-B, 和Nogo-C)，它们有相同的羧基端188个氨基酸序列。Nogo-A是一个跨膜蛋白，富集在内质网中并在中枢神经系统中高表达，而在骨骼肌和心脏肌肉中低表达(1-3)。随着年龄增长脑部发育中Nogo-A的表达下降。成人中枢神经系统，神经元生长的负调控能使中枢神经系统在重塑过程中稳定。Nogo-A和nogo受体1(NgR1)，p75神经营养因子受体p75NTR，以及跨膜蛋白LINGO1蛋白，一起介导神经突生长抑制。Nogo受体信号通路复合物激活RhoA/ROCK通路，这将在神经系统脑外伤后破坏神经元生长锥和抑制轴突生长。研究提示抑制Nogo A对外伤性脑损伤有益。Nogo-B和Nogo-C抑制BACE1活性和淀粉样前体蛋白加工，提示它们在细胞存活中扮演了重要角色(4)。

1. Chen, M.S. et al. (2000) Nature 403, 434-9.
2. Prinjha, R. et al. (2000) Nature 403, 383-4.
3. GrandPré, T. et al. (2002) Nature 417, 547-51.
4. Schwab, M.E. (2010) Nat Rev Neurosci 11, 799-811.

原厂资料：

Specificity / Sensitivity

Nogo-A Antibody recognizes endogenous levels of total Nogo-A protein. Based on sequence homology, this antibody is not expected to recognize Nogo-B or Nogo-C.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly220 of human Nogo-A protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Neurite outgrowth inhibition protein (Nogo, RTN4) is a reticulon family protein that was identified as an axonal growth inhibitor of the central nervous system (CNS). Nogo occurs as three major isoforms (Nogo-A, Nogo-B, and Nogo-C) that share a common carboxy terminus of 188 amino acids. Nogo-A is transmembrane protein enriched in the endoplasmic reticulum and expressed at high levels in the CNS, and more weakly in skeletal and heart muscle (1-3). Expression of Nogo-A decreases with increasing age during brain development. In the adult CNS, negative regulation of neuronal growth leads to stabilization of the CNS wiring at the expense of extensive plastic rearrangements. Nogo-A meditates inhibition of neurite growth together with the nogo receptor 1 (NgR1), the p75 neurotrophin receptor p75NTR, and the transmembrane LINGO1 protein. This Nogo receptor signaling complex activates the RhoA/ROCK pathway, which collapses neuronal growth cones and inhibits axonal growth in the CNS following traumatic brain injury. Research studies suggest that inhibition of Nogo A may be beneficial to patients with traumatic brain injury. Nogo-B and Nogo-C inhibit BACE1 activity and amyloid precursor protein processing, suggesting a role in cell survival (4).

1. Chen, M.S. et al. (2000) Nature 403, 434-9.
2. Prinjha, R. et al. (2000) Nature 403, 383-4.
3. GrandPré, T. et al. (2002) Nature 417, 547-51.
4. Schwab, M.E. (2010) Nat Rev Neurosci 11, 799-811.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody