Phospho-4E-BP1 (Thr70) (D7F6I) Rabbit mAb

• 产品编号：CST-13396S      品牌：CST       原厂货号：13396S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Specificity / Sensitivity

Phospho-4E-BP1 (Thr70) (D7F6I) Rabbit mAb recognizes endogenous levels of 4E-BP1 protein only when phosphorylated at Ser70.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr70 of human 4E-BP1 protein.

原厂资料：

Specificity / Sensitivity

Phospho-4E-BP1 (Thr70) (D7F6I) Rabbit mAb recognizes endogenous levels of 4E-BP1 protein only when phosphorylated at Ser70.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr70 of human 4E-BP1 protein.

Background

Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5).

1. Pause, A. et al. (1994) Nature 371, 762-7.
2. Brunn, G.J. et al. (1997) Science 277, 99-101.
3. Gingras, A.C. et al. (1998) Genes Dev 12, 502-13.
4. Fadden, P. et al. (1997) J Biol Chem 272, 10240-7.
5. Gingras, A.C. et al. (1999) Genes Dev 13, 1422-37.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.