Artemis (D7O8V) Rabbit mAb

• 产品编号：CST-13381S      品牌：CST       原厂货号：13381S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Artemis (D7O8V) Rabbit mAb兔单抗以识别内源性总的artemis蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与人artemis的Pro367邻近残基序列一致。DNA双链损伤（DSBs）是潜在的有害损伤区域可能由电离辐射（IR），拟辐射化合物，或DNA复制抑制因子所引发。细胞通过两种不同但可能有交叉的信号通路感知并修复DSBs，非同源末端连接（NHEJ）和同源重组（HR）。通过HR途径的DNA修复限制在细胞周期的S和G2期，而NHEJ可以发生在任何时期。两种途径的缺陷都和包括癌症在内的人类疾病相关（1）。 Artemis是一个广泛表达的带有核酸内切酶功能的NHEJ因子。Artemis在DNA修复中通过促进非同源行末端连接（2）以及通过ATM/ATR通路控制细胞周期检验点(3)发挥功能。NHEJ机制也在V(D)J重组中发挥作用，这是一个在免疫球蛋白和T细胞受体基因中引发多样性的过程，artemis是该过程中的一个重要因子（4,5）。Artemis基因发生突变与人类放射敏感型重症联合免疫缺陷（SCID）相关。

1. Hartlerode, A.J. and Scully, R. (2009) Biochem J 423, 157-68.
2. Kurosawa, A. et al. (2013) PLoS One 8, e72253.
3. Zhang, X. et al. (2004) Mol Cell Biol 24, 9207-20.
4. Mansilla-Soto, J. and Cortes, P. (2003) J Exp Med 197, 543-7.
5. Ma, Y. et al. (2002) Cell 108, 781-94.
6. Noordzij, J.G. et al. (2003) Blood 101, 1446-52.
7. Li, L. et al. (2002) J Immunol 168, 6323-9.

原厂资料：

Specificity / Sensitivity

Artemis (D7O8V) Rabbit mAb recognizes endogenous levels of total artemis protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro367 of human artemis protein

Background

DNA double-strand breaks (DSBs) are potentially hazardous lesions that can be induced by ionizing radiation (IR), radiomimetic chemicals, or DNA replication inhibitors. Cells recognize and repair DSBs via two distinct but partly overlapping signaling pathways, nonhomologous end joining (NHEJ) and homologous recombination (HR). DNA repair via the HR pathway is restricted to S and G2 phases of the cell cycle, while NHEJ can occur during any phase. Defects in both pathways have been associated with human disease, including cancer (1).

Artemis is a ubiquitously expressed NHEJ factor that exhibits endonuclease activity. Artemis functions in DNA repair by promoting nonhomologous end joining (2), as well as in cell cycle checkpoint control through ATM/ATR signaling (3).

NHEJ machinery is also utilized in V(D)J recombination, a process that generates diversity in immunoglobulin and T cell receptor genes, and artemis is a key factor in this process (4,5). Mutations in the corresponding artemis gene (DCLRE1C) are associated with a radiosensitive type of severe combined immunodeficiency (SCID) in humans (6,7).

1. Hartlerode, A.J. and Scully, R. (2009) Biochem J 423, 157-68.
2. Kurosawa, A. et al. (2013) PLoS One 8, e72253.
3. Zhang, X. et al. (2004) Mol Cell Biol 24, 9207-20.
4. Mansilla-Soto, J. and Cortes, P. (2003) J Exp Med 197, 543-7.
5. Ma, Y. et al. (2002) Cell 108, 781-94.
6. Noordzij, J.G. et al. (2003) Blood 101, 1446-52.
7. Li, L. et al. (2002) J Immunol 168, 6323-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.