NUP98 (C39A3) Rabbit mAb?(Alexa Fluor??488 Conjugate)

• 产品编号：CST-13358S      品牌：CST       原厂货号：13358S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

</p> NUP98 (C39A3) Rabbit mAb (Alexa Fluor^® 488 Conjugate) 可以识别内源性总的NUP98蛋白。</p>单克隆抗体由合成肽段免疫动物产生，该肽段与人NUP98蛋白Pro671附近氨基酸一致。</p> Cell Signaling Technology抗体被Alexa Fluor®488荧光染料标记，经过对人细胞免疫分析的内部试验验证。经证实抗体与未标记的NUP98 (C39A3) Rabbit mAb #2598有同样的反应性。</p>Nucleoporin 98 kDa (NUP98)是细胞核孔复合物的一部分。会表达三个不同的前体再通过自剪切形成一个98kDa氨基末端肽段（NUP98）和6羧基端，96 (NUP96)和88（p88）kDa肽段（1,2）。NUP98在它的氨基末端含有FG和GLFG重复结构域，并在羧基端还有一个RNA结合结构域（3）。NUP98基因位于染色体的11p15.5,该区域在白血病中经常发生重排。迄今为止，NUP98共发现了15种融合配体（4,5）。

1. Fontoura, B.M. et al. (1999) J Cell Biol 144, 1097-112.
2. Rosenblum, J.S. and Blobel, G. (1999) Proc Natl Acad Sci U S A 96, 11370-5.
3. Griffis, E.R. et al. (2004) Mol Biol Cell 15, 1991-2002.
4. Slape, C. and Aplan, P.D. (2004) Leuk Lymphoma 45, 1341-50.
5. Nakamura, T. (2005) Int J Hematol 82, 21-7.

原厂资料：

Specificity / Sensitivity

<p>NUP98 (C39A3) Rabbit mAb (Alexa Fluor^® 488 Conjugate) detects endogenous levels of total NUP98 protein.

Source / Purification

<p>Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro671 of human NUP98.

Description

<p>This Cell Signaling Technology antibody is conjugated to Alexa Fluor^® 488 fluorescent dye and tested in-house for direct immunofluorescence analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated NUP98 (C39A3) Rabbit mAb # 2598.

Background

<p>Nucleoporin 98 kDa (NUP98) is a component of the nuclear pore complex. It is expressed as three different precursors that undergo auto-cleavage to generate a common amino-terminal 98 kDa peptide (NUP98) and carboxy-terminal 6, 96 (NUP96) and 88 (p88) kDa peptides (1,2). NUP98 contains FG and GLFG repeat domains at its amino terminus and a RNA-binding domain in its carboxy terminus (3). The NUP98 gene is localized on chromosome 11p15.5, a region frequently rearranged in leukemias. To date, 15 fusion partners have been identified for NUP98 (4,5).

1. Fontoura, B.M. et al. (1999) J Cell Biol 144, 1097-112.
2. Rosenblum, J.S. and Blobel, G. (1999) Proc Natl Acad Sci U S A 96, 11370-5.
3. Griffis, E.R. et al. (2004) Mol Biol Cell 15, 1991-2002.
4. Slape, C. and Aplan, P.D. (2004) Leuk Lymphoma 45, 1341-50.
5. Nakamura, T. (2005) Int J Hematol 82, 21-7.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibod-
ies. Protect from light. Do not freeze.