CDK6 (D4S8S) Rabbit mAb

• 产品编号：CST-13331S      品牌：CST       原厂货号：13331S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CDK6 (D4S8S) Rabbit mAb兔单抗以识别内源性总的CDK6蛋白。该抗体与其他CDK蛋白没有交叉反应性。单克隆抗体由合成肽段免疫动物产生，该肽段与人CDK6的羧基端邻近残基序列一致。细胞周期蛋白依赖性激酶可以和它们的周期蛋白伴侣和CDK抑制剂结合。CDK6和CDK4和D-型细胞周期蛋白结合，靶向成视网膜细胞瘤蛋白，促进细胞通过G1/S期限制点（1）。CDK6/cyclin D复合物和一个INK4 CDK抑制剂家族成员结合使复合物进入失活状态（2,3）。不像CDK抑制剂的INK4家族，CDK抑制剂p21 Waf1/Cip1可以加强CDK4和CDK6与它们的cyclin D伴侣的结合（4）。

1. Lukas, J. et al. (1996) Mol Cell Biol 16, 6917-25.
2. Hirai, H. et al. (1995) Mol Cell Biol 15, 2672-81.
3. Pestell, R.G. et al. (1999) Endocr Rev 20, 501-34.
4. Flores-Rozas, H. et al. (1994) Proc Natl Acad Sci U S A 91, 8655-9.

原厂资料：

Specificity / Sensitivity

CDK6 (D4S8S) Rabbit mAb recognizes endogenous levels of total CDK6 protein. This antibody does not cross-react with other CDK proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CDK6 protein.

Background

The cyclin-dependent kinases form complexes with their cyclin partners and with CDK inhibitors. CDK6 and CDK4 associate with the D-type cyclins and target the retinoblastoma protein, allowing passage through the G1/S phase restriction point (1). CDK6/cyclin D complexes are sequestered in their inactive form through binding to one of the INK4 CDK inhibitor family members (2,3). Unlike the INK4 family of CDK inhibitors, the CDK inhibitor p21 Waf1/Cip1 may enhance the association of CDK4 and CDK6 with their cyclin D partners (4).

1. Lukas, J. et al. (1996) Mol Cell Biol 16, 6917-25.
2. Hirai, H. et al. (1995) Mol Cell Biol 15, 2672-81.
3. Pestell, R.G. et al. (1999) Endocr Rev 20, 501-34.
4. Flores-Rozas, H. et al. (1994) Proc Natl Acad Sci U S A 91, 8655-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody