APC1 (D1E9D) Rabbit mAb

• 产品编号：CST-13329S      品牌：CST       原厂货号：13329S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

APC1 (D1E9D) Rabbit mAb兔单抗识别内源性的APC1总蛋白。单克隆抗体经合成与人APC1蛋白羰基末端邻近的氨基酸残基序列一致的肽段，免疫动物产生。所有真核细胞的细胞增殖严格依赖于泛素连接酶（E3）APC/C的活性，它的主要功能在于激发细胞周期由分裂中期向分裂后期的转变。APC/C是在分裂间期细胞核中发现的一种1.5 MDa蛋白复合物。这一复合物在细胞质种分散，并在有丝分裂中与部分纺锤体有关。APC/C通过促进底物蛋白聚泛素链的组装而发挥多种功能，主要是通过26S蛋白酶体使这些蛋白降解(1,2)。在人类，12种不同的APC/C亚基已经被确定。像所有E3酶一样，APC/C利用已经被E1酶活化的泛素，然后转化为E2酶。确实，APC/C已经被证实短暂地与UBCH5和UBCH10 E2酶相互作用，部分经过包含有环指结构域的亚基APC11 (3-5)。除外E2酶，APC/C的活性严格地依赖于一些辅助因素，与APC/C所处的细胞周期特殊阶段有关。最好的研究是有关Cdc20 和Cdh1/FZR1，包含有C-末端WD40结构域，并通过这些底物(6)中特殊的识别元件D-boxes (7) 和KEN-boxes (8)参与APC/C底物的识别。促细胞分裂复合体亚基1(APC1，Anaphase-Promoting Complex Subunit 1)是最大的APC / C亚基并且广泛表达(9)。尽管功能意义尚不清楚,研究表明, 在真核细胞周期的有丝分裂阶段，APC1大部分的丝氨酸和苏氨酸残基被磷酸化(10)。

1. Qiao, X. et al. (2010) Cell Cycle 9, 3904-12.
2. Harper, J.W. et al. (2002) Genes Dev 16, 2179-206.
3. Chang, L. et al. (2014) Nature 513, 388-93.
4. Carroll, C.W. and Morgan, D.O. (2002) Nat Cell Biol 4, 880-7.
5. Gmachl, M. et al. (2000) Proc Natl Acad Sci U S A 97, 8973-8.
6. Leverson, J.D. et al. (2000) Mol Biol Cell 11, 2315-25.
7. Kraft, C. et al. (2005) Mol Cell 18, 543-53.
8. Glotzer, M. et al. (1991) Nature 349, 132-8.
9. Pfleger, C.M. and Kirschner, M.W. (2000) Genes Dev 14, 655-65.
10. Jörgensen, P.M. et al. (2001) Gene 262, 51-9.
11. Kraft, C. et al. (2003) EMBO J 22, 6598-609.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Dog, Pig, Horse

Specificity / Sensitivity

APC1 (D1E9D) Rabbit mAb recognizes endogenous levels of total APC1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human APC1 protein.

Background

Cell proliferation in all eukaryotic cells depends strictly upon the ubiquitin ligase (E3) activity of the anaphase promoting complex/cyclosome (APC/C), whose main function is to trigger the transition of the cell cycle from metaphase to anaphase. APC/C is a 1.5 MDa protein complex found in the nucleus of interphase cells. This complex diffuses throughout the cytoplasm and associates with parts of the spindle apparatus during mitosis. APC/C performs its various functions by promoting the assembly of polyubiquitin chains on substrate proteins, which targets these proteins for degradation by the 26S proteasome (1,2). In humans, twelve different APC/C subunits have been identified. Like all E3 enzymes, APC/C utilizes ubiquitin residues that have been activated by E1 enzymes and then transferred to E2 enzymes. Indeed APC/C has been shown to transiently interact with UBCH5 and UBCH10 E2 enzymes, in part, via the RING-finger domain-containing subunit, APC11 (3-5). In addition to E2 enzymes, APC/C activity is also strictly dependent upon one of several cofactors that associate with APC/C during specific phases of the cell cycle. The best studied of these are Cdc20 and Cdh1, which contain a carboxy-terminal WD40 domain and participate in the recognition of APC/C substrates by interacting with specific recognition elements in these substrates (6), called D-boxes (7) and KEN-boxes (8).Anaphase-Promoting Complex Subunit 1 (APC1) is the largest subunit of the APC/C and is ubiquitously expressed (9). Although the functional significance is unclear, research studies have demonstrated that APC1 undergoes extensive phosphorylation on serine and threonine residues during the mitotic phase of the eukaryotic cell cycle (10).

1. Qiao, X. et al. (2010) Cell Cycle 9, 3904-12.
2. Harper, J.W. et al. (2002) Genes Dev 16, 2179-206.
3. Chang, L. et al. (2014) Nature 513, 388-93.
4. Carroll, C.W. and Morgan, D.O. (2002) Nat Cell Biol 4, 880-7.
5. Gmachl, M. et al. (2000) Proc Natl Acad Sci U S A 97, 8973-8.
6. Leverson, J.D. et al. (2000) Mol Biol Cell 11, 2315-25.
7. Kraft, C. et al. (2005) Mol Cell 18, 543-53.
8. Glotzer, M. et al. (1991) Nature 349, 132-8.
9. Pfleger, C.M. and Kirschner, M.W. (2000) Genes Dev 14, 655-65.
10. Jörgensen, P.M. et al. (2001) Gene 262, 51-9.
11. Kraft, C. et al. (2003) EMBO J 22, 6598-609.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody