SignalSilence? PP1α siRNA I

• 产品编号：CST-13319S      品牌：CST       原厂货号：13319S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

SignalSilence^® PP1α siRNA I抑制人，小鼠和大鼠的PP1α表达。Cell Signaling Technology (CST) 公司的SignalSilence® PP1α siRNA I帮助研究人员使用RNA干扰技术特异性抑制PP1α表达，该技术通过向细胞内导入双链RNA分子以选择性的抑制某些基因的表达。所有CST公司的SignalSilence® siRNA都经过了严格的内部测试，western分析显示能够能够降低目标蛋白的表达。寡核苷酸合成经过了三苯甲基的逐碱基分析以确保耦合效率。随后经过亲和固相萃取纯化。退火的RNA双链在经过质谱分析以确保双链的准确性。新批次都会经过质谱分析与之前的批次进行比对以保证不同批次之间的稳定性。CST建议转染100 nM SignalSilence® PP1α siRNA I，48-72小时后裂解细胞。转染的步骤请参阅转染试剂的说明书。关于使用问题，可以随时咨询CST。 每管试剂能进行100次转染，该转染次数以总体积为300μl 的1个24孔板孔中siRNA终浓度100 nM来进行计算。 1型蛋白磷酸酶（PP1），一种丝氨酸/苏氨酸磷酸酶，在真核细胞中高度保守。发现了4种PP1亚型：PP1α, PP1δ, PP1γ1和 PP1γ2 (1).PP1的一种生物学功能包括参与细胞周期调控(1). 例如PP1在有丝分裂时去磷酸化Rb和cdc25（2,3）。Cdc2激酶以细胞周期依赖的方式磷酸化PP1α的Thr320会抑制PP1α活性（4）。

1. Rubin, E. et al. (1998) Front Biosci 3, D1209-19.
2. Durfee, T. et al. (1993) Genes Dev 7, 555-69.
3. Izumi, T. et al. (1992) Mol Biol Cell 3, 927-39.
4. Kwon, Y.G. et al. (1997) Proc Natl Acad Sci U S A 94, 2168-73.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat

Specificity / Sensitivity

SignalSilence^® PP1α siRNA I inhibits human, mouse and rat PP1α expression.

Description

SignalSilence^® PP1α siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit PP1α expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence^® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 100 nM SignalSilence^® PP1α siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.

Background

Type 1 protein phosphatase (PP1), a serine/threonine phosphatase, is highly conserved in eukaryotic cells. Four isoforms of PP1 have been characterized: PP1α, PP1δ, PP1γ1 and PP1γ2 (1). Involvement in cell cycle regulation is one of the biological functions of PP1 (1). It has been illustrated that PP1 dephosphorylates Rb and cdc25 during mitosis (2,3). A cell cycle-dependent phosphorylation at Thr320 of PP1α by cdc2 kinase inhibits PP1α activity (4).

1. Rubin, E. et al. (1998) Front Biosci 3, D1209-19.
2. Durfee, T. et al. (1993) Genes Dev 7, 555-69.
3. Izumi, T. et al. (1992) Mol Biol Cell 3, 927-39.
4. Kwon, Y.G. et al. (1997) Proc Natl Acad Sci U S A 94, 2168-73.

注意事项：

Storage: PP1α siRNA I is supplied in RNAse-free water. Aliquot
and store at -20ºC.