Complexin-1/2 Antibody

• 产品编号：CST-13302S      品牌：Cell Signaling Technology       原厂货号：13302S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Complexin-1/2 Antibody 兔多抗可以识别内源性的complexin-1和complexin-2总蛋白。该多克隆抗体由合成肽段免疫动物产生，该肽段与人complexin-2蛋白羧基末端附近氨基酸一致。抗体由蛋白A和多肽亲和层析纯化得到。Complexins是由氨基和羧基末端结构域的非结构的结构域包围的α螺旋结构域组成的小的可溶性蛋白(1)。这些胞浆蛋白和t-SNAREs以低亲和度结合而以高亲和性募集SNARE复合物(1,2)。神经细胞表达两种亚型(3)，complexin-1和 complexin-2，以调节诱发和自发的胞吐作用(4,5)。RNAi介导的敲除(6)或基因沉默(7)改变了complexin的表达水平导致自发融合时间和神经递质释放过程的变化，这反映complexin在一致性和刺激性神经突出中都发挥作用。

1. Chen, X. et al. (2002) Neuron 33, 397-409.
2. McMahon, H.T. et al. (1995) Cell 83, 111-9.
3. Reim, K. et al. (2005) J Cell Biol 169, 669-80.
4. Reim, K. et al. (2001) Cell 104, 71-81.
5. Huntwork, S. and Littleton, J.T. (2007) Nat Neurosci 10, 1235-7.
6. Maximov, A. et al. (2009) Science 323, 516-21.
7. Xue, M. et al. (2008) Proc Natl Acad Sci U S A 105, 7875-80.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

Complexin-1/2 Antibody recognizes endogenous levels of total complexin-1 and complexin-2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human complexin-2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Complexins are small soluble proteins composed of a central α-helical-structured domain surrounded by amino- and carboxy-terminal unstructured domains (1). These cytosolic proteins bind to t-SNAREs with low affinity and to assembled SNARE complexes with high affinity (1,2). Two isoforms, complexin-1 and complexin-2, are expressed in neuronal cells (3) where they regulate evoked and spontaneous exocytosis (4,5). Altered complexin expression resulting from RNAi-mediated knockdown (6) or gene invalidation (7) leads to alteration in spontaneous fusion events and neurotransmitter release, which reflects functions at both inhibitory and stimulatory synapses.

1. Chen, X. et al. (2002) Neuron 33, 397-409.
2. McMahon, H.T. et al. (1995) Cell 83, 111-9.
3. Reim, K. et al. (2005) J Cell Biol 169, 669-80.
4. Reim, K. et al. (2001) Cell 104, 71-81.
5. Huntwork, S. and Littleton, J.T. (2007) Nat Neurosci 10, 1235-7.
6. Maximov, A. et al. (2009) Science 323, 516-21.
7. Xue, M. et al. (2008) Proc Natl Acad Sci U S A 105, 7875-80.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.