SAV1 (D6M6X) Rabbit mAb

• 产品编号：CST-13301S      品牌：CST       原厂货号：13301S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

SAV1 (D6M6X) Rabbit mAb兔单抗可以识别内源性的总SAV1蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与人SAV1蛋白羧基末端临近的氨基酸残基序列一致。Salvador homolog (SAV1)，原名WW45，最初被鉴定为含有一对WW结构域和一个卷曲螺旋区域的45 kDa蛋白(1)。 SAV1随后被证明能够充当一个支架蛋白，作为蛋白质复合物，其中包含激酶MST2和LATS1和转录共激活因子YAP (2)。该蛋白复合物包括了Hippo信号通路的核心成分，调控的主要细胞功能包括细胞接触抑制和凋亡，这些功能可以调控组织生长和器官大小(3,4)。对果蝇进行基因扫描发现了SAV1在细胞周期调控和凋亡中的作用(5)，而缺少Sav1的胎鼠表现出增生和上皮细胞亢进分化的现象(6)。这些发现，连同一些人癌细胞系中SAV1发生突变的现象，提示SAV1可以作为Hippo信号通路的肿瘤抑制因子(5,7)。

1. Valverde, P. (2000) Biochem Biophys Res Commun 276, 990-8.
2. Oka, T. et al. (2008) J Biol Chem 283, 27534-46.
3. Guo, C. et al. (2007) Curr Biol 17, 700-5.
4. Zeng, Q. and Hong, W. (2008) Cancer Cell 13, 188-92.
5. Tapon, N. et al. (2002) Cell 110, 467-78.
6. Lee, J.H. et al. (2008) EMBO J 27, 1231-42.
7. Donninger, H. et al. (2011) J Biol Chem 286, 18483-91.

原厂资料：

Specificity / Sensitivity

SAV1 (D6M6X) Rabbit mAb recognizes endogenous levels of total SAV1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human SAV1 protein.

Background

Salvador homolog (SAV1), originally named WW45, was first identified as a 45 kDa protein containing a pair of WW domains and a coiled-coil region (1). SAV1 was subsequently shown to function as a scaffold protein, in a protein complex that includes the kinases MST2 and LATS1, and the transcriptional co-activator YAP (2). This protein complex comprises the core components of the Hippo signaling pathway, which regulates important cellular functions, including contact inhibition and apoptosis, that function to regulate tissue growth and organ size (3,4). A genetic screen inDrosophila identified a role for SAV1 in cell cycle regulation and apoptosis (5), while embryonic mice lacking Sav1 displayed hyperplastic growth and epithelial differentiation effects (6). These findings, together with the observation that SAV1 is mutated a number of human cancer cell lines, suggest that SAV1 functions as a tumor suppressor in the Hippo signaling pathway (5, 7).

1. Valverde, P. (2000) Biochem Biophys Res Commun 276, 990-8.
2. Oka, T. et al. (2008) J Biol Chem 283, 27534-46.
3. Guo, C. et al. (2007) Curr Biol 17, 700-5.
4. Zeng, Q. and Hong, W. (2008) Cancer Cell 13, 188-92.
5. Tapon, N. et al. (2002) Cell 110, 467-78.
6. Lee, J.H. et al. (2008) EMBO J 27, 1231-42.
7. Donninger, H. et al. (2011) J Biol Chem 286, 18483-91.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.