CNOT3 (E1L9S) Rabbit mAb

• 产品编号：CST-13300S      品牌：CST       原厂货号：13300S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CNOT3 (E1L9S) Rabbit mAb能够检测内源性的CNOT3总蛋白水平。该单克隆抗体是用围绕人CNOT3 蛋白Leu638位点的氨基酸序列合成的多肽免疫动物而生产的。进化保守的CCR4-NOT (CNOT) 复合物能够调控真核细胞中的mRNA代谢（1）。这种调控作用发生在mRNA合成和降解的不同水平，包括转录起始，延伸，去乙酰化以及降解（1）。已经鉴定出了该复合物中存在的多种组分包括CNOT1, CNOT2, CNOT3, CNOT4, CNOT6, CNOT7, CNOT8, CNOT9, 以及CNOT10（2）。另外，该复合物的亚基组成在不同的组织中是各异的（3）。研究发现CNOT3 (与CNOT1和 CNOT2一起)能够抑制早期发育转录因子的表达，以帮助维持小鼠和人胚胎干细胞（ES）特征（4）。 还有研究表明，CNOT3可以使有丝分裂纺锤体蛋白MAD1 mRNA去稳定化，因此在有丝分裂进程中具有重要作用（5）。此外CNOT3可作为修饰基因来影响引起常染色体显性色素性视网膜炎（6）的突变的外显率。并可在成人T细胞急性淋巴细胞白血病中作为肿瘤抑制因子（7）。

1. Denis, C.L. and Chen, J. (2003) Prog Nucleic Acid Res Mol Biol 73, 221-50.
2. Lau, N.C. et al. (2009) Biochem J 422, 443-53.
3. Chen, C. et al. (2011) Biochem Biophys Res Commun 411, 360-4.
4. Zheng, X. et al. (2012) Stem Cells 30, 910-22.
5. Takahashi, A. et al. (2012) Biochem Biophys Res Commun 419, 268-73.
6. Venturini, G. et al. (2012) PLoS Genet 8, e1003040.
7. De Keersmaecker, K. et al. (2013) Nat Genet 45, 186-90.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat

Specificity / Sensitivity

CNOT3 (E1L9S) Rabbit mAb recognizes endogenous levels of total CNOT3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu638 of human CNOT3 protein.

Background

The evolutionarily conserved CCR4-NOT (CNOT) complex regulates mRNA metabolism in eukaryotic cells (1). This regulation occurs at different levels of mRNA synthesis and degradation, including transcription initiation, elongation, deadenylation, and degradation (1). Multiple components, including CNOT1, CNOT2, CNOT3, CNOT4, CNOT6, CNOT6L, CNOT7, CNOT8, CNOT9, and CNOT10 have been identified in this complex (2). In addition, subunit composition of this complex has been shown to vary among different tissues (3).Research studies indicate that CNOT3 (along with CNOT1 and CNOT2) represses early developmental transcription factor expression, helping to maintain embryonic stem (ES) cell identity in mice and humans (4). Additional studies suggest that CNOT3 plays a role in mitotic progression as it destabilizes mitotic spindle assembly protein MAD1 mRNA (5). Finally, CNOT3 appears to act as a modifier gene affecting the penetrance of mutations causing autosomal dominant retinitis pigmentosa (6) and as a tumor suppressor associated with cases of adult T-cell acute lymphoblastic leukemia (7).

1. Denis, C.L. and Chen, J. (2003) Prog Nucleic Acid Res Mol Biol 73, 221-50.
2. Lau, N.C. et al. (2009) Biochem J 422, 443-53.
3. Chen, C. et al. (2011) Biochem Biophys Res Commun 411, 360-4.
4. Zheng, X. et al. (2012) Stem Cells 30, 910-22.
5. Takahashi, A. et al. (2012) Biochem Biophys Res Commun 419, 268-73.
6. Venturini, G. et al. (2012) PLoS Genet 8, e1003040.
7. De Keersmaecker, K. et al. (2013) Nat Genet 45, 186-90.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.